Biotin-PEG3-Azide

Name: Biotin-PEG3-Azide
Brand: Tocris Bioscience
SKU: 7524

Tocris Bioscience, part of Bio-Techne | Catalog # 7524

COA / SDS

Biotin with PEG linker and reactive click handle

Key Product Details

Description:	Biotin with PEG linker and reactive click handle
Chemical Name:	(3aS,4S,6aR)-N-[2-[2-[2-(2-Azidoethoxy)ethoxy]ethoxy]ethyl]hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide
Purity:	≥95% (HPLC)
Molecular Weight:	444.55

View Full Technical Details for Biotin-PEG3-Azide

Technical Data
Citations

Biological Activity for Biotin-PEG3-Azide

Biotin-PEG3-Azide is a biotinylation reagent for labeling alkyne-containing biomolecules either via Cu(I)-catalyzed Azide-Alkyne Click Chemistry reaction (CuAAC) or via Cu(I)-free Strain-Promoted Alkyne-Azide Click Chemistry (SPAAC) reaction with cyclooctyne derivatives.

Technical Data for Biotin-PEG3-Azide

M. Wt	444.55
Formula	C₁₈H₃₂N₆O₅S
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	875770-34-6
PubChem ID	60146223
InChi Key	ZWFOOMQCYIGZBE-ZOBUZTSGSA-N
Smiles	C(CCCC(NCCOCCOCCOCCN=[N+]=[N-])=O)[C@H]1[C@@]2([C@](CS1)(NC(=O)N2)[H])[H]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Biotin-PEG3-Azide

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	44.45	100
	water	44.45	100

Preparing Stock Solutions for Biotin-PEG3-Azide

The following data is based on the product molecular weight 444.55.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.25 mL	11.25 mL	22.49 mL
5 mM	0.45 mL	2.25 mL	4.50 mL
10 mM	0.22 mL	1.12 mL	2.25 mL
50 mM	0.04 mL	0.22 mL	0.45 mL

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Reconstitution Calculator

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References for Biotin-PEG3-Azide

References are publications that support the biological activity of the product.

Zhang Use of NAD tagSeq II to identify growth phase-dependent alterations in E. coli RNA NAD⁺ capping. Proc.Natl.Acad.Sci.U.S.A. 2021 PMID: 33782135