5-Ethynyluridine

Name: 5-Ethynyluridine
Brand: Tocris Bioscience
SKU: 7206

Tocris Bioscience, part of Bio-Techne | Catalog # 7206

COA / SDS

Uridine analog, used for labeling RNA synthesis

Key Product Details

Description:	Uridine analog, used for labeling RNA synthesis
Chemical Name:	1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-ethynylpyrimidine-2,4(1H,3H)-dione
Purity:	≥98% (HPLC)
Molecular Weight:	268.23

View Full Technical Details for 5-Ethynyluridine

Technical Data
Citations

Biological Activity for 5-Ethynyluridine

5-Ethynyluridine is a cell-permeable uridine analog. 5-Ethynyluridine can be used in vitro and in vivo to label newly synthesized RNA. 5-Ethynyluridine can be used to study changes in transcription.

Technical Data for 5-Ethynyluridine

M. Wt	268.23
Formula	C₁₁H₁₂N₂O₆
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	69075-42-9
PubChem ID	14885960
InChi Key	QCWBIPKYTBFWHH-FDDDBJFASA-N
Smiles	OC[C@H]1O[C@@H](N2C=C(C(NC2=O)=O)C#C)[C@@H]([C@@H]1O)O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for 5-Ethynyluridine

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	13.41	50
	DMSO	26.82	100

Preparing Stock Solutions for 5-Ethynyluridine

The following data is based on the product molecular weight 268.23.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.73 mL	18.64 mL	37.28 mL
5 mM	0.75 mL	3.73 mL	7.46 mL
10 mM	0.37 mL	1.86 mL	3.73 mL
50 mM	0.07 mL	0.37 mL	0.75 mL

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Reconstitution Calculator

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References for 5-Ethynyluridine

References are publications that support the biological activity of the product.

Van't Sant In vivo 5-ethynyluridine (EU) labelling detects reduced transcription in Purkinje cell degeneration mouse mutants, but can itself induce neurodegeneration. Acta Neuropathol.Commun. 2021 PMID: 34020718
Nainar An optimized chemical-genetic method for cell-specific metabolic labeling of RNA. Nat.Methods 2020 PMID: 32015544