2A3

Product Description

2A3 is an electrophilic chemical probe that acylates the 2'-OH in the RNA backbone. 2A3 can be used for RNA SHAPE-MaP experiments in cell lines or live bacteria. It labels single-stranded or flexible regions more than folded regions (SHAPE methodology) and enables analysis of RNA secondary structures at single nucleotide resolution. For intracellular experiments, 2A3 has higher signal-to-noise ratio and prediction accuracy than NAI (Cat. No. 7004). 2A3 is cell permeable, and can probe both gram-positive and gram-negative bacteria.

Licensing Information

Sold with kind permission of Dr Danny Incarnato

Solubility

Preparing Stock Solutions for 2A3

The following data is based on the product molecular weight 188.19

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

3 2 1 Recalculate

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	5.31 mL	26.57 mL	53.14 mL
5 mM	1.06 mL	5.31 mL	10.63 mL
10 mM	0.53 mL	2.66 mL	5.31 mL
50 mM	0.11 mL	0.53 mL	1.06 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Marinus A novel SHAPE reagent enables the analysis of RNA structure in living cells with unprecedented accuracy. Nucleic Acids Res. 2021 PMID: 33398343