WZ 4003
Tocris Bioscience, part of Bio-Techne | Catalog # 5177
Potent and selective NUAK1/2 inhibitor
Product Description
WZ 4003 is a potent and selective NUAK1/2 inhibitor (IC50 values are 20 and 100 nM respectively). Exhibits no significant inhibition against a panel of 139 kinases, including ten AMPK family members. Inhibits NUAK1-mediated MYPT1 phosphorylation. Also inhibits cell proliferation in U2OS cells.External Portal Information
Chemicalprobes.org is a portal that offers independent guidance on the selection and/or application of small molecules for research. The use of WZ 4003 is reviewed on the chemical probes website.Product Specifications
Molecular Weight
496.99
Formula
C25H29ClN6O3
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
N-3-[[5-Chloro-2-[[2-methoxy-4-(4-methyl-1-piperazinyl)phenyl]amino]-4-pyrimidinyl]oxy]phenyl]propanamide
CAS Number
1214265-58-3
PubChem ID
72200024
InChI Key
SDGJBAUIGHSMRI-UHFFFAOYSA-N
SMILES
CN(CC3)CCN3C(C=C2)=CC(OC)=C2NC1=NC=C(Cl)C(OC4=CC(NC(CC)=O)=CC=C4)=N1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| 1eq. HCl | 24.85 | 50 | |
| DMSO | 9.94 | 20 with gentle warming |
Preparing Stock Solutions
for WZ 4003
The following data is based on the product molecular weight 496.99
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 4.02 mL | 20.12 mL | 40.24 mL |
| 2.5 mM | 0.80 mL | 4.02 mL | 8.05 mL |
| 5 mM | 0.40 mL | 2.01 mL | 4.02 mL |
| 25 mM | 0.08 mL | 0.40 mL | 0.80 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Banerjee Interplay between Polo kinase, LKB1-activated NUAK1 kinase, PP1βMYPT1 phosphatase complex and the SCFβTrCP E3 ubiquitin ligase. Biochem.J. 2014 PMID: 24785407
- Banerjee Characterization of WZ4003 and HTH-01-015 as selective inhibitors of the LKB1-tumour-suppressor-activated NUAK kinases. Biochem.J. 2014 PMID: 24171924
- Zhou Novel mutant-selective EGFR kinase inhibitors against EGFR T790M. Nature 2009 PMID: 20033049
Product Specific Notices for WZ 4003
For research use only
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