TPPB
Tocris Bioscience, part of Bio-Techne | Catalog # 5343
High affinity PKC activator; also APP modulator
Key Product Details
Description
High affinity PKC activator; also APP modulator
Product Description
TPPB is a high affinity and cell permeable protein kinase C activator (Ki =11.9 nM), which induces differentiation of hESCs into Pdx-1 expressing pancreatic progenitor cells. TPPB also enhances the non-amyloidgenic processing of amyloid precursor protein, increasing secretion of sAPPα.Product Specifications
Molecular Weight
501.54
Formula
C27H30F3N3O3
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
(2E,4E)-N-[(2S,5S)-1,2,3,4,5,6-Hexahydro-5-(hydroxymethyl)-1-methyl-2-(1-methylethyl)-3-oxo-1,4-benzodiazocin-8-yl]-5-[4-(trifluoromethyl)phenyl]-2,4-pentadienamide
CAS Number
497259-23-1
PubChem ID
9935767
InChI Key
WOLVEMPZUIFSII-IHHOKICGSA-N
SMILES
CC([C@@H]1N(C2=CC=C(C=C2C[C@H](NC1=O)CO)NC(/C=C/C=C/C3=CC=C(C(F)(F)F)C=C3)=O)C)C
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 25.08 | 50 |
Preparing Stock Solutions
for TPPB
The following data is based on the product molecular weight 501.54
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 3.99 mL | 19.94 mL | 39.88 mL |
| 2.5 mM | 0.80 mL | 3.99 mL | 7.98 mL |
| 5 mM | 0.40 mL | 1.99 mL | 3.99 mL |
| 25 mM | 0.08 mL | 0.40 mL | 0.80 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Chen A small molecule that directs differentiation of human ESCs into the pancreatic lineage. Nat.Chem.Biol. 2009 PMID: 19287398
- Kozikowski New amide-bearing benzolactam-based protein kinase C modulators induce enhanced secretion of the amyloid precursor protein metabolite sAPPα. J.Med.Chem. 2003 PMID: 12540236
- Rezania Reversal of diabetes with Ins-producing cells derived in vitro from human pluripotent stem cells. Nat.Biotechnol. 2014 PMID: 25211370
Product Specific Notices for TPPB
For research use only
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