Thapsigargin

Name: Thapsigargin
Brand: Tocris Bioscience
SKU: 1138
Rating: 4 (7 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 1138

(7)

Citations (460)

COA / SDS

Potent inhibitor of SERCA ATPase

Key Product Details

Description:	Potent inhibitor of SERCA ATPase
Chemical Name:	(3S,3aR,4S,6S,6AR,7S,8S,9bS)-6-(Acetyloxy)-2,3,3a,4,5,6,6a,7,8,9b-decahydro-3,3a-dihydroxy-3,6,9-trimethyl-8-[[(2Z)-2-methyl-1-oxo-2-butenyl]oxy]-2-oxo-4-(1-oxobutoxy)azuleno[4,5-b]furan-7-yl octanoate
Purity:	≥97% (HPLC)
Molecular Weight:	650.76

View Full Technical Details for Thapsigargin

Technical Data
Citations / Reviews (7)

Biological Activity for Thapsigargin

Thapsigargin is a potent inhibitor of sarco/endoplasmic reticulum Ca²⁺-ATPases (SERCA) that causes endoplasmic reticulum (ER) stress. Thapsigargin can be used to induce autophagy in mammalian cells. In vitro, thapsigargin blocks infection of immortilized and primary human cells with respiratory syncytial virus (RSV), SARS-CoV-2 and influenza A. Thapsigargin protects mice against lethal influenza A viral infection and reduces virus titres in the lungs of treated mice.

Technical Data for Thapsigargin

M. Wt	650.76
Formula	C₃₄H₅₀O₁₂
Storage	Store at -20°C
Purity	≥97% (HPLC)
CAS Number	67526-95-8
PubChem ID	446378
InChi Key	IXFPJGBNCFXKPI-FSIHEZPISA-N
Smiles	O=C(O[C@@H]2[C@@]3(O)[C@@H](OC([C@](C)3O)=O)C1=C(C)[C@H](OC(/C(C)=C\C)=O)[C@@H](OC(CCCCCCC)=O)[C@@]([H])1[C@](OC(C)=O)(C)C2)CCC

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Thapsigargin

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	65.08	100

Preparing Stock Solutions for Thapsigargin

The following data is based on the product molecular weight 650.76.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.54 mL	7.68 mL	15.37 mL
5 mM	0.31 mL	1.54 mL	3.07 mL
10 mM	0.15 mL	0.77 mL	1.54 mL
50 mM	0.03 mL	0.15 mL	0.31 mL

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References for Thapsigargin

References are publications that support the biological activity of the product.

Goulding Thapsigargin at non-cytotoxic levels induces a potent host antiviral response that blocks influenza A virus replication. Viruses 2020 PMID: 32992478
Al-Beltagi Thapsigargin is a broad-Spectrum inhibitor of major human respiratory viruses: coronavirus, respiratory syncytial virus and influenza A virus. Viruses 2021 PMID: 33546185
Ding Differential effects of endoplasmic reticulum stress-induced autophagy on cell survival. J.Biol.Chem. 2007 PMID: 17135238
Yu Specific substitutions at amino acid 256 of the sarcoplasmic/endoplasmic reticulum Ca²⁺ transport ATPase mediate resistance to thapsigargin in thapsigargin-resistant hamster cells. J.Biol.Chem. 1998 PMID: 9452480
Treiman A tool coming of age: thapsigargin as an inhibitor of sarco-endoplasmic reticulum Ca²⁺-ATPases. TiPS 1998 PMID: 9612087
Davidson and Varhol Kinetics of thapsigargin-Ca²⁺-ATPase (sarcoplasmic reticulum) interaction reveals a two-step binding mechanism and picomolar inhibition. J.Biol.Chem. 1995 PMID: 7744817