TBB

Name: TBB
Brand: Tocris Bioscience
SKU: 2275

Tocris Bioscience, part of Bio-Techne | Catalog # 2275

Citations (37)

COA / SDS

Selective cell-permeable CK2 inhibitor

Key Product Details

Description:	Selective cell-permeable CK2 inhibitor
Alternative Names:	NSC 231634
Chemical Name:	4,5,6,7-Tetrabromobenzotriazole
Purity:	≥98% (HPLC)
Molecular Weight:	434.71

View Full Technical Details for TBB

Technical Data
Citations

Biological Activity for TBB

TBB is a cell-permeable, selective inhibitor of casein kinase-2 (CK2) (IC₅₀ = 0.9 and 1.6 μM for rat liver and human recombinant CK2 respectively). Exhibits modest discrimination between CK2 subunits, with K_i values ranging from 80 nM to 210 nM. Acts in an ATP/GTP-competitive manner and displays one to two orders of magnitude selectivity over a panel of 33 protein kinases.

Compound Libraries for TBB

TBB is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Kinase Inhibitor Library and Tocriscreen Antiviral Library. Find out more about compound libraries available from Tocris.

Technical Data for TBB

M. Wt	434.71
Formula	C₆HBr₄N₃
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	17374-26-4
PubChem ID	1694
InChi Key	OMZYUVOATZSGJY-UHFFFAOYSA-N
Smiles	BrC1=C2NN=NC2=C(Br)C(Br)=C1Br

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for TBB

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	43.47	100

Preparing Stock Solutions for TBB

The following data is based on the product molecular weight 434.71.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.30 mL	11.50 mL	23.00 mL
5 mM	0.46 mL	2.30 mL	4.60 mL
10 mM	0.23 mL	1.15 mL	2.30 mL
50 mM	0.05 mL	0.23 mL	0.46 mL

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Reconstitution Calculator

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References for TBB

References are publications that support the biological activity of the product.

Pagano Optimization of protein kinase CK2 inhibitors derived from 4,5,6,7-tetrabromobenzimidazole. J.Med.Chem. 2004 PMID: 15566294
Zien Tetrabromobenzotriazole (TBBt) and tetrabromobenzimidazole (TBBz) as selective inhibitors of protein kinase CK2: evaluation of their effects on cells and different molecular forms of human CK2. Biochim.Biophys.Acta. 2005 PMID: 16203192
Sarno Selectivity of 4,5,6,7-tetrabromobenzotriazole, an ATP site-directed inhibitor of protein kinase CK2 ('casein kinase-2'). FEBS Lett. 2001 PMID: 11343704

TBB