SU 5402

Tocris Bioscience, part of Bio-Techne | Catalog # 3300

COA / SDS

Potent FGFR and VEGFR inhibitor

Key Product Details

Description:	Potent FGFR and VEGFR inhibitor
Alternative Names:	PF-02969207,PNU-0290908
Chemical Name:	2-[(1,2-Dihydro-2-oxo-3H-indol-3-ylidene)methyl]-4-methyl-1H-pyrrole-3-propanoic acid
Purity:	≥95% (HPLC)
Molecular Weight:	296.32

View Full Technical Details for SU 5402

Technical Data
Citations

Biological Activity for SU 5402

SU 5402 is a potent and selective vascular endothelial growth factor receptor (VEGFR) and fibroblast growth factor receptor (FGFR) inhibitor (IC₅₀ values are 0.02, 0.03, 0.51 and > 100 μM at VEGFR2, FGFR1, PDGFRβ and EGFR respectively). Inhibits embryonic left-right determination and exhibits potent anticancer activity in vitro and in vivo. Also attenuates integrin β4-induced differentiation of neural stem cells. Supports mESC self-renewal. Can be used in a small molecule cocktail to generate cortical neurons from hiPSCs (see our protocol below).

For more information about how SU 5402 may be used, see our protocol: Accelerated Induction of Cortical Neurons from hiPSCs

Technical Data for SU 5402

M. Wt	296.32
Formula	C₁₇H₁₆N₂O₃
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	215543-92-3
PubChem ID	5289418
InChi Key	JNDVEAXZWJIOKB-JYRVWZFOSA-N
Smiles	CC1=CNC(\C=C(C2=CC=CC=C2N3)/C3=O)=C1CCC(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for SU 5402

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	29.63	100

Preparing Stock Solutions for SU 5402

The following data is based on the product molecular weight 296.32.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.37 mL	16.87 mL	33.75 mL
5 mM	0.67 mL	3.37 mL	6.75 mL
10 mM	0.34 mL	1.69 mL	3.37 mL
50 mM	0.07 mL	0.34 mL	0.67 mL

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Reconstitution Calculator

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References for SU 5402

References are publications that support the biological activity of the product.

Buehr Capture of authentic embryonic stem cells from rat blastocysts. Cell 2008 PMID: 19109897
Su Neural stem cell differentiation is mediated by integrin beta4 in vitro. Int.J.Biochem.Cell Biol. 2009 PMID: 18834954
Paterson Preclinical studies of fibroblast growth factor receptor 3 as a therapeutic target in multiple myeloma. Br.J.Haematol. 2004 PMID: 14871245
Tanaka FGF-induced vesicular release of sonic hedgehog and retinoic acid in leftward nodal flow is critical for left-right determination. Nature 2005 PMID: 15889083
Sun Design, synthesis and evaluations of substituted 3-[(3- or 4-carboxyethylpyrrol-2-yl)methylidenyl]indolin-2-ones as inhibitors of VEGF, FGF and PDGF receptor tyrosine kinases. J.Med.Chem. 1999 PMID: 10602697