SMBA 1

Name: SMBA 1
Brand: Tocris Bioscience
SKU: 5314

Tocris Bioscience, part of Bio-Techne | Catalog # 5314

Citations (3)

COA / SDS

High affinity and selective activator of Bax

Key Product Details

Description:	High affinity and selective activator of Bax
Chemical Name:	2-[(2-Nitro-9H-fluorene-9-ylidene)methyl]phenol
Purity:	≥98% (HPLC)
Molecular Weight:	315.32

View Full Technical Details for SMBA 1

Technical Data
Citations

Biological Activity for SMBA 1

SMBA 1 is a high affinity and selective activator of Bax (K_i = 43.3 nM). Binds to the S184 binding pocket to block Bax phosphorylation. Does not bind to other Bcl-2 family members, including Bcl-2, Bak and Bid. Facilitates Bax insertion into the mitochondrial membrane and promotes Bax-dependent cytochrome c release. Induces apoptosis in lung cancer cell lines expressing high levels of Bax selectively over normal cells expressing low levels of Bax. Represses tumor growth of A549 lung cancer xenografts in mice.

Technical Data for SMBA 1

M. Wt	315.32
Formula	C₂₀H₁₃NO₃
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	906440-37-7
PubChem ID	349156
InChi Key	PEYKRJBXACUOOB-UHFFFAOYSA-N
Smiles	OC(C=CC=C4)=C4\C=C2C3=C(C=CC([N+]([O-])=O)=C3)C1=CC=CC=C1/2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for SMBA 1

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	31.53	100
	ethanol	3.15	10 with gentle warming

Preparing Stock Solutions for SMBA 1

The following data is based on the product molecular weight 315.32.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.17 mL	15.86 mL	31.71 mL
5 mM	0.63 mL	3.17 mL	6.34 mL
10 mM	0.32 mL	1.59 mL	3.17 mL
50 mM	0.06 mL	0.32 mL	0.63 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for SMBA 1

References are publications that support the biological activity of the product.

Xin Small-molecule Bax agonists for cancer therapy. Nat.Commun. 2014 PMID: 25230299