sCy5DA
Tocris Bioscience, part of Bio-Techne | Catalog # 7834
FDAA for super-resolution microscopy of bacteria
Key Product Details
Description
FDAA for super-resolution microscopy of bacteria
Product Description
sCy5DA is a fluorescent D-amino acid. It is suitable for labeling peptidoglycans in live bacteria. It is efficiently incorporated into the peptidoglycans of a wide variety of bacteria, including gram-positive, gram-negative, and mycobacteria. Incorporates particularly well into gram-negative C. crescentus. sCy5DA labelled bacteria can be imaged by single molecule localization microscopy (SMLM; also referred to as PALM or STORM). Excitation/emission maxima = 646/665 nm.Scientific Data Images for sCy5DA
Single molecule localization microscopy (SMLM) image of Vibrio cholerae cells labeled with 500 μM sCy5DA
Single molecule localization microscopy (SMLM) image of Bacillus subtilis cells labeled with 100 μM sCy5DA
Single molecule localization microscopy (SMLM) image of Mycobacterium smegmatis cells labeled with 100 μM sCy5DA
Optical Data
| Emission Color | Red |
| λabs | 646 nm |
| λem | 665 nm |
| Extinction Coefficient (ε) | 197,240 M-1cm-1 |
| Cell Permeable | Yes |
| Application | STORM |
Product Specifications
Molecular Weight
728.88
Formula
C35H44N4O9S2
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
(R)-2-Ammonio-3-(6-((E)-3,3-dimethyl-5-sulfo-2-((2E,4E)-5-(1,3,3-trimethyl-5-sulfonato-3H-indol-1-ium-2-yl)penta-2,4-dien-1-ylidene)indolin-1-yl)hexanamido)propanoate
InChI Key
ZSTSSXALPFZNBO-HHHXNRCGSA-N
SMILES
CC1(C)C2=C(C=CC(S(=O)(O)=O)=C2)N(CCCCCC(NC[C@H](C([O-])=O)[NH3+])=O)/C1=C/C=C/C=C/C3=[N+](C4=C(C3(C)C)C=C(S(=O)([O-])=O)C=C4)C
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 7.29 | 10 |
Preparing Stock Solutions
for sCy5DA
The following data is based on the product molecular weight 728.88
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 13.72 mL | 68.60 mL | 137.20 mL |
| 0.5 mM | 2.74 mL | 13.72 mL | 27.44 mL |
| 1 mM | 1.37 mL | 6.86 mL | 13.72 mL |
| 5 mM | 0.27 mL | 1.37 mL | 2.74 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Zhang Fluorescent D-amino acids for super-resolution microscopy of the bacterial cell wall. ACS Chem.Biol. 2022 PMID: 35994360
Product Specific Notices for sCy5DA
For research use only
Loading...
Loading...
Loading...