SAHA

Product Description

SAHA inhibits Class I and II histone deacetylases (HDACs); induces accumulation of acetylated histones H2A, H2B, H3 and H4 in transformed cultured cells. Suppresses cell growth in a range of cancer cell lines; induces apoptosis in cutaneous T cell lymphoma cells in vitro. Activates autophagy. SAHA increases efficiency of transcription factor-induced reprogramming of mouse embryonic fibroblasts (MEF) to induced pluripotent stem cells (iPSC). Enhances adeno-associated virus transduction of HeLa cells. Also induces activation and replication of latent HIV in vitro.

Solubility

Preparing Stock Solutions for SAHA

The following data is based on the product molecular weight 264.32

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.78 mL	18.92 mL	37.83 mL
5 mM	0.76 mL	3.78 mL	7.57 mL
10 mM	0.38 mL	1.89 mL	3.78 mL
50 mM	0.08 mL	0.38 mL	0.76 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Nicolson Identification and validation of small molecules that enhance recombinant adeno-associated virus transduction following high-throughput screens. J.Virol. 2016 PMID: 27147738
• Narasipura Epigenetic regulation of HIV-1 latency in astrocytes. J.Virol. 2014 PMID: 24352441
• Galluzzi Pharmacological modulation of autophagy: therapeutic potential and persisting obstacles. Nat.Rev.Drug.Discov. 2017 PMID: 28529316
• Huangfu Induction of pluripotent stem cells by defined factors is greatly improved by small-molecule compounds. Nat.Biotechnol. 2008 PMID: 18568017
• Marks and Breslow Dimethyl sulfoxide to vorinostat: development of this histone deacetylase inhibitor as an anticancer drug. Nat.Biotechnol. 2007 PMID: 17211407
• Leoni The antitumor histone deacetylase inhibitor suberoylanilide hydroxamic acid exhibits antiinflammatory properties via suppression of cytokines. Proc.Natl.Acad.Sci.U.S.A 2002 PMID: 11867742
• Butler Suberoylanilide hydroxamic acid, an inhibitor of histone deacetylase, suppresses the growth of prostate cancer cells in vitro and in vivo. Cancer Res. 2000 PMID: 11016644