Ro 31-8220 mesylate
Tocris Bioscience, part of Bio-Techne | Catalog # 2002
Protein kinase inhibitor
Key Product Details
Description
Protein kinase inhibitor
Alternative Names
Bisindolylmaleimide IX
Product Description
Ro 31-8220 mesylate is a protein kinase C inhibitor, with activity at other protein kinases (IC50 values are 33, 3, 8, 15 and 38 nM for PKCα, MAPKAP-K1b, MSK1, GSK3β and S6K1 respectively). Activates JNK and glycogen synthase, and inhibits MAPK and ERK2, in rat adipocytes and L6 myotubes. Also inhibits voltage-dependent Na+ channels in the micromolar range. Induces protein-protein interaction between mutant SMAD4R361H and SMAD3, leading to reactivation of dormant SMAD4R361H-mediated transcriptional activity and restoration of TGF-β-induced tumor suppression activity in SMAD4 mutant cancer cells.Product Specifications
Molecular Weight
553.65
Formula
C25H23N5O2S.CH3SO3H
Storage
Desiccate at -20°C
Purity
≥97% (HPLC)
Chemical Name
3-[3-[2,5-Dihydro-4-(1-methyl-1H-indol-3-yl)-2,5-dioxo-1H-pyrrol-3-yl]-1H-indol-1-yl]propyl carbamimidothioic acid ester mesylate
CAS Number
138489-18-6
PubChem ID
11628205
InChI Key
SAWVGDJBSPLRRB-UHFFFAOYSA-N
SMILES
CS(O)(=O)=O.CN1C=C(C2=CC=CC=C12)C1=C(C(=O)NC1=O)C1=CN(CCCSC(N)=N)C2=CC=CC=C12
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 55.37 | 100 | |
| Ethanol | 2.77 | 5 |
Preparing Stock Solutions
for Ro 31-8220 mesylate
The following data is based on the product molecular weight 553.65
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.81 mL | 9.03 mL | 18.06 mL |
| 5 mM | 0.36 mL | 1.81 mL | 3.61 mL |
| 10 mM | 0.18 mL | 0.90 mL | 1.81 mL |
| 50 mM | 0.04 mL | 0.18 mL | 0.36 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Tang Hypomorph mutation-directed small-molecule protein-protein interaction inducers to restore mutant SMAD4-suppressed TGF-β signaling. Cell Chem.Biol. 2020 PMID: 33326750
- Standaert RO 31-8220 activates c-Jun N-terminal kinase and glycogen synthase in rat adipocytes and L6 myotubes. Comparison to actions of Ins. Endocrinology 1999 PMID: 10218965
- Lingameneni Inhibition of voltage-dependent sodium channels by Ro 31-8220, a 'specific' protein kinase C inhibitor. FEBS Lett. 2000 PMID: 10812087
- Davies Specificity and mechanism of action of some commonly used protein kinase inhibitors. Biochem.J. 2000 PMID: 10998351
- Davis Potent, selective inhibitors of protein kinase C. FEBS Lett. 1989 PMID: 2532156
Product Specific Notices for Ro 31-8220 mesylate
For research use only
Loading...
Loading...