RO-275

Name: RO-275
Brand: Tocris Bioscience
SKU: 8977

Tocris Bioscience, part of Bio-Techne | Catalog # 8977

COA / SDS

Potent and selective HCN1 inhibitor

Key Product Details

Description:	Potent and selective HCN1 inhibitor
Chemical Name:	4-((2-Chloro-3-methylpyridin-4-yl)ethynyl)-5-methyl-1-(pyridin-3-yl)-1H-imidazole-2-carboxamide
Purity:	≥98% (HPLC)
Molecular Weight:	351.79

View Full Technical Details for RO-275

Technical Data
Citations

Biological Activity for RO-275

RO-275 is a potent and selective HCN1 (hyperpolarization-activated cyclic-nucleotide-gated channel 1) inhibitor (IC₅₀ = 46 nM). It shows <100-fold selectivity over HCN2, HCN3 and HCN4. In vivo, RO-275 improves performance in a translationally relevant touchscreen task of working memory (TUNL) in rats. RO-275 is orally bioavailable and brain-penetrant.

Licensing Information

This compound is supplied in conjunction with the Structural Genomics Consortium. For further characterization details, please visit the RO-275 probe summary on the SGC website.

Technical Data for RO-275

M. Wt	351.79
Formula	C₁₈H₁₄ClN₅O
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	2648837-04-9
PubChem ID	156441547
InChi Key	STQKUEFQEVDNLU-UHFFFAOYSA-N
Smiles	O=C(C1=NC(C#CC2=C(C)C(Cl)=NC=C2)=C(C)N1C3=CC=CN=C3)N

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for RO-275

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	17.59	50

Preparing Stock Solutions for RO-275

The following data is based on the product molecular weight 351.79.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	5.69 mL	28.43 mL	56.85 mL
2.5 mM	1.14 mL	5.69 mL	11.37 mL
5 mM	0.57 mL	2.84 mL	5.69 mL
25 mM	0.11 mL	0.57 mL	1.14 mL

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References for RO-275

References are publications that support the biological activity of the product.

Harde Selective and brain-penetrant HCN1 inhibitors reveal links between synaptic integration, cortical function, and working memory. Cell.Chem.Biol. 2024 PMID: 38042151