Psoralen-triethylene glycol azide

Product Description

Psoralen TEG Azide is used to probe RNA structure and conformation in live cells, using the cross-linking of matched RNAs and deep sequencing or COMRADES method. Psoralen crosslinks the RNA and the azide enables subsequent click reaction with biotin. The COMRADES method has been used to determine the architecture of the Zika virus RNA genome inside cells, and identified multiple site-specific interactions with human noncoding RNAs. It has also been used to map RNA-RNA interactions along the SARS-CoV-2 genome and subgenomes inside cells. Psoralen TEG Azide is cell permeable. For detailed methods please see references section below.

Solubility

Preparing Stock Solutions for Psoralen-triethylene glycol azide

The following data is based on the product molecular weight 458.52

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.18 mL	10.90 mL	21.81 mL
5 mM	0.44 mL	2.18 mL	4.36 mL
10 mM	0.22 mL	1.09 mL	2.18 mL
50 mM	0.04 mL	0.22 mL	0.44 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Ziv COMRADES determines in vivo RNA structures and interactions. Nat. Methods 2018 PMID: 30202058
• Ziv The short- and long-range RNA-RNA interactome of SARS-CoV-2. Mol. Cell 2020 PMID: 33259809