Pladienolide B
Tocris Bioscience, part of Bio-Techne | Catalog # 6070
mRNA splicing inhibitor; antitumor
Key Product Details
Description
mRNA splicing inhibitor; antitumor
Product Description
Pladienolide B is a mRNA splicing inhibitor that decreases splicing capacity up to 75% in vitro. Pladienolide B directly targets splicesome-associated 130 (SAP130), inhibits splicing factor 3B subunit (SF3B1) and impairs U2 small nuclear ribonucleoprotein (U2 snRNP) interaction with pre-mRNA. Pladienolide B arrests the cell cycle in G1 and G2/M phases and displays antitumor activity against gastric cancer cells (IC50 values are 1.6-4.9 nM). Transient treatment with pladienolide B can reprogram pluripotent hESCs into a distinct type of zigotic genome activation (ZGA)-like cells (ZLCs). Cell permeable and active in vivo.Product Specifications
Molecular Weight
536.70
Formula
C30H48O8
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
(4R,7R,8S,9E,11S,12S)-8-(Acetyloxy)-4,7-dihydroxy-12-[(1E,3E,5S)-6-[(2R,3R)-3-[(1R,2S)-2-hydroxy-1-methylbutyl]-2-oxiranyl]-1,5-dimethyl-1,3-hexadien-1-yl]-7,11-dimethyloxacyclododec-9-en-2-one
CAS Number
445493-23-2
PubChem ID
90909513
InChI Key
SDOUORKJIJYJNW-UHFFFAOYSA-N
SMILES
C[C@@H](/C=C/[C@H](OC(C)=O)[C@](C)(O)CC[C@@H](O)C1)[C@@H](/C(C)=C/C=C/[C@@H](C)C[C@@H]2[C@@H]([C@H](C)[C@@H](O)CC)O2)OC1=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 1 |
Preparing Stock Solutions
for Pladienolide B
The following data is based on the product molecular weight 536.70
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.02 mM | 93.16 mL | 465.81 mL | 931.62 mL |
| 0.1 mM | 18.63 mL | 93.16 mL | 186.32 mL |
| 0.2 mM | 9.32 mL | 46.58 mL | 93.16 mL |
| 1 mM | 1.86 mL | 9.32 mL | 18.63 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Li Capturing totipotency in human cells through spliceosomal repression. Cell 2024 PMID: 38843832
- Pederiva Splicing controls the ubiquitin response during DNA double-strand break repair. Cell Death Differ. 2016 PMID: 27315300
- Sato High antitumor activity of pladienolide B and its derivative in gastric cancer. Cancer Sci. 2014 PMID: 24635824
- Lee Therapeutic targeting of splicing in cancer. Nature Medicine
Product Specific Notices for Pladienolide B
For research use only
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