Pemetrexed
Tocris Bioscience, part of Bio-Techne | Catalog # 6185
Thymidylate synthetase inhibitor, also inhibits dihydrofolate reductase, GARFT and AICART
Key Product Details
Description
Thymidylate synthetase inhibitor, also inhibits dihydrofolate reductase, GARFT and AICART
Product Description
Pemetrexed is a high affinity dihydrofolate reductase inhibitor (Ki = 7 nM). Also inhibits thymidylate synthase, AICART and glycinamide ribonucleotide formyltransferase (Ki values are 109 nM, and 3.5 μM and 9.3 μM, respectively). Indirectly activates AMPK. Inhibits proliferation of cancer cell lines in vitro.Product Specifications
Molecular Weight
427.41
Formula
C20H21N5O6
Storage
Store at +4°C
Purity
≥98% (HPLC)
Chemical Name
N-[4-[2-(2-Amino-4,7-dihydro-4-oxo-3H-pyrrolo[2,3-d]pyrimidin-5-yl)ethyl]benzoyl]-L-glutamic acid
CAS Number
137281-23-3
PubChem ID
394493
InChI Key
WBXPDJSOTKVWSJ-UHFFFAOYSA-N
SMILES
NC1=NC(C2=C(N1)NC=C2CCC3=CC=C(C(N[C@H](C(O)=O)CCC(O)=O)=O)C=C3)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 42.74 | 100 |
Preparing Stock Solutions
for Pemetrexed
The following data is based on the product molecular weight 427.41
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.34 mL | 11.70 mL | 23.40 mL |
| 5 mM | 0.47 mL | 2.34 mL | 4.68 mL |
| 10 mM | 0.23 mL | 1.17 mL | 2.34 mL |
| 50 mM | 0.05 mL | 0.23 mL | 0.47 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Rothbart Pemetrexed indirectly activates the metabolic kinase AMPK in human carcinomas. Cancer Res. 2010 PMID: 21159649
- Shih LY231514, a pyrrolo[2,3-d]pyrimidine-based antifolate that inhibits multiple folate-requiring enzymes. Cancer Res. 1997 PMID: 9067281
Product Specific Notices for Pemetrexed
For research use only
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