PEI STAR™ transfection reagent

Tocris Bioscience, part of Bio-Techne | Catalog # 7854

Polyethylenimine (PEI) transfection reagent, chemically-defined
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Key Product Details

Description

Polyethylenimine (PEI) transfection reagent, chemically-defined

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Product Description

PEI STAR™ is a chemically-defined, high-performance polyethylenimine (PEI) transfection reagent for cost-effective, affordable and scalable transient gene expression. PEI is a synthetic polymer with an exceptionally high positive charge density in pH-neutral solutions. Positively charged PEI binds strongly to negatively charged DNA and imparts a net cationic charge, allowing the DNA to enter cells. PEI is a non-viral vector commonly used to transfect HEK293 and CHO cells. Applications include production of recombinant proteins, antibodies and viruses.

Pre-dissolved, ready-to-use formulation of PEI STAR™ also available: PEI STAR-Go™ transfection reagent (Cat. No. 8174).

For more information on how to use PEI STAR™ transfection reagent, see our protocols: PEI STAR™ Transfection Reagent Preparation, Gene Expression in Adherent HEK293 Cells, Gene Expression in CHO Suspensions, rAAV Production in Adherent HEK293 Cells, Gene Expression in HEK293 Suspensions, Lentivirus Production with PEI STAR™, rAAV Production in Suspension HEK293 Cells.

PEI STAR is a trademark of Bio-Techne Corp.

Scientific Data Images for PEI STAR™ transfection reagent

Application of PEI STAR™ transfection reagent

Application of PEI STAR™ transfection reagent.

PEI STAR™ performance data comparison (HEK293): HEK 293 - 20 mL cultures containing HEK293 suspensions were transfected with a CMV-SEAP plasmid at optimized PEI/DNA ratios using either PEI STAR™ (3:1) or leading competitor PEI. SEAP expression levels were quantified 5 days post-transfection using phosphatase reporter dye and UV/Vis absorbance.
Application of PEI STAR™ transfection reagent

Application of PEI STAR™ transfection reagent.

PEI STAR™ performance data comparison (CHO): CHO - 20 mL cultures containing CHO suspensions were transfected with a CMV-SEAP plasmid at optimized PEI/DNA ratios using either PEI STAR™ (5:1) or leading competitor PEI. SEAP expression levels were quantified 5 days post-transfection using phosphatase reporter dye and UV/Vis absorbance.
Cells transfected with a reporter GFP construct using a leading competitor PEI and PEI STAR™

Transfection of a reporter GFP construct using a leading competitor PEI and PEI STAR™.

HEK293 cells grown in DMEM/10% FBS to 75% confluency in a 24 well plate. 1 μg of DNA + 25 μL optimem and 2 μg of PEI (1 mg/mL) + 25 μL optimem were incubated for 8 minutes before addition to cells. PEI STAR™ showed significantly higher expression of GFP in imaged cells.

Product Specifications

Storage

Store at RT

Chemical Name

Poly[imino(1,2-ethanediyl)] hydrochloride

CAS Number

49553-93-7

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

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Product Documents for PEI STAR™ transfection reagent

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PEI STAR™ transfection reagent

For research use only

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