PD 407824
Tocris Bioscience, part of Bio-Techne | Catalog # 2694
Potent and selective inhibitor of Chk1 and Wee1
Key Product Details
Description
Potent and selective inhibitor of Chk1 and Wee1
Product Description
PD 407824 is a potent and selective inhibitor of checkpoint kinases Chk1 and Wee1 (IC50 values are 47 and 97 nM respectively). Displays selectivity over a range of other protein kinases; IC50 values are 3.4, 3.75, > 5, > 50, > 50 and > 50 μM for PKC, CDK4, other CDKs, c-Src, PDGFR and FGFR respectively. Also sensitizes hESCs to BMP4 to induce differentiation.Licensing Information
Sold for research purposes under agreement from Pfizer Inc.Product Specifications
Molecular Weight
328.32
Formula
C20H12N2O3
Storage
Store at +4°C
Purity
≥98% (HPLC)
Chemical Name
9-Hydroxy-4-phenyl-pyrrolo[3,4-c]carbazole-1,3(2H,6H)-dione
CAS Number
622864-54-4
PubChem ID
4369491
InChI Key
IAUZTOZLTFSMIE-UHFFFAOYSA-N
SMILES
OC1=CC2=C(NC3=C2C2=C(C(=O)NC2=O)C(=C3)C2=CC=CC=C2)C=C1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 32.83 | 100 | |
| Ethanol | 8.2 | 25 |
Preparing Stock Solutions
for PD 407824
The following data is based on the product molecular weight 328.32
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.05 mL | 15.23 mL | 30.46 mL |
| 5 mM | 0.61 mL | 3.05 mL | 6.09 mL |
| 10 mM | 0.30 mL | 1.52 mL | 3.05 mL |
| 50 mM | 0.06 mL | 0.30 mL | 0.61 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Palmer 4-Phenylpyrrolo[3,4-c]carbazole-1,3(2H,6H)-dione inhibitors of the checkpoint kinase Wee1. Structure-activity relationships for chromophore modification and phenylring substitutions. J.Med.Chem. 2006 PMID: 16884302
- Feng Discovery of a small-molecule BMP sensitizer for human embryonic stem cell differentiation. Cell Rep. 2016 PMID: 27210748
- Squire Structure and inhibition of the human cell cycle checkpoint kinase, Wee1A kinase: an atypical tyrosine kinase with a key role in CDK1 regulation. Structure 2005 PMID: 15837193
Product Specific Notices for PD 407824
For research use only
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