PD 166285 dihydrochloride

Name: PD 166285 dihydrochloride
Brand: Tocris Bioscience
SKU: 3785

Tocris Bioscience, part of Bio-Techne | Catalog # 3785

Citations (6)

COA / SDS

Potent Src inhibitor; also inhibits FGFR1, PDGFRβ and Wee1

Key Product Details

Description:	Potent Src inhibitor; also inhibits FGFR1, PDGFRβ and Wee1
Chemical Name:	6-(2,6-Dichlorophenyl)-2-[[4-[2-(diethylamino)ethoxy]phenyl]amino]-8-methylpyrido[2,3-d]pyrimidin-7(8H)-one dihydrochloride
Purity:	≥98% (HPLC)
Molecular Weight:	585.35

View Full Technical Details for PD 166285 dihydrochloride

Technical Data
Citations

Biological Activity for PD 166285 dihydrochloride

PD 166285 dihydrochloride is a potent inhibitor of the tyrosine kinases c-Src, fibroblast growth factor receptor 1 (FGFR1), and platelet-derived growth factor receptor β (PDGFRβ) (IC₅₀ values are 8.4, 39.3 and 98.3 nM respectively). Also inhibits the checkpoint kinases Wee1 and Myt1; abolishes Cdc2 phosphorylation in numerous tumor cell lines and abrogates the G₂ checkpoint.

Licensing Information

Sold for research purposes under agreement from Pfizer Inc.

Compound Libraries for PD 166285 dihydrochloride

PD 166285 dihydrochloride is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Kinase Inhibitor Library and Tocriscreen Antiviral Library. Find out more about compound libraries available from Tocris.

Technical Data for PD 166285 dihydrochloride

M. Wt	585.35
Formula	C₂₆H₂₇Cl₂N₅O₂.2HCl
Storage	Desiccate at RT
Purity	≥98% (HPLC)
CAS Number	212391-63-4
PubChem ID	9916391
InChi Key	NADLBPWBFGTESN-UHFFFAOYSA-N
Smiles	CN(C1=NC(NC4=CC=C(OCCN(CC)CC)C=C4)=NC=C1C=C2C3=C(Cl)C=CC=C3Cl)C2=O.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for PD 166285 dihydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	58.53	100

Preparing Stock Solutions for PD 166285 dihydrochloride

The following data is based on the product molecular weight 585.35.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.71 mL	8.54 mL	17.08 mL
5 mM	0.34 mL	1.71 mL	3.42 mL
10 mM	0.17 mL	0.85 mL	1.71 mL
50 mM	0.03 mL	0.17 mL	0.34 mL

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References for PD 166285 dihydrochloride

References are publications that support the biological activity of the product.

Wang Radiosensitization of p53 mutant cells by PD0166285, a novel G₂ checkpoint abrogator. Cancer Res. 2001 PMID: 11719452
Hashimoto Cell cycle regulation by the Wee1 inhibitor PD0166285, Pyrido [2,3-d] pyimidine, in the B16 mouse melanoma cell line. BMC Cancer 2006 PMID: 17177986
Panek In vitro pharmacological characterization of PD 166285, a new nanomolar potent and broadly active protein tyrosine kinase inhibitor. J.Pharmacol.Exp.Ther. 1997 PMID: 9400019