PAC 1

Name: PAC 1
Brand: Tocris Bioscience
SKU: 2581

Tocris Bioscience, part of Bio-Techne | Catalog # 2581

Citations (15)

COA / SDS

Activator of procaspase-3; pro-apoptotic

Key Product Details

Description:	Activator of procaspase-3; pro-apoptotic
Chemical Name:	4-(Phenylmethyl)-1-piperazineacetic acid [[2-hydroxy-3-(2-propenyl)phenyl]methylene]hydrazide
Purity:	≥98% (HPLC)
Molecular Weight:	392.5

View Full Technical Details for PAC 1

Technical Data
Citations

Biological Activity for PAC 1

PAC 1 is a procaspase-activating compound; activates procaspase-3 to produce caspase-3 (EC₅₀ = 0.22 μM). Also activates procaspase-7 in a less efficient manner (EC₅₀ = 4.5 μM). Pro-apoptotic; induces apoptosis in both cancerous and non-cancerous cells dependent on procaspase-3 concentration (IC₅₀ values are 0.003 - 1.41 and 5.02 - 9.98 μM respectively).

Technical Data for PAC 1

M. Wt	392.5
Formula	C₂₃H₂₈N₄O₂
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	315183-21-2
PubChem ID	9675990
InChi Key	YQNRVGJCPCNMKT-LFVJCYFKSA-N
Smiles	O=C(N/N=C/C3=CC=CC(CC=C)=C3O)CN(CC2)CCN2CC1=CC=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for PAC 1

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	1eq. HCl	29.44	75
	DMSO	39.25	100

Preparing Stock Solutions for PAC 1

The following data is based on the product molecular weight 392.5.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.55 mL	12.74 mL	25.48 mL
5 mM	0.51 mL	2.55 mL	5.10 mL
10 mM	0.25 mL	1.27 mL	2.55 mL
50 mM	0.05 mL	0.25 mL	0.51 mL

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Reconstitution Calculator

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References for PAC 1

References are publications that support the biological activity of the product.

Peterson Procaspase-3 activation as an anti-cancer strategy: structure-activity relationship of procaspase-activating compound 1 (PAC-1) and its cellular co-localization with caspase-3. J.Med.Chem. 2009 PMID: 19708658
Putt Small-molecule activation of procaspase-3 to caspase-3 as a personalized anticancer strategy. Nat.Chem.Biol. 2006 PMID: 16936720