ONO AE3 208

Name: ONO AE3 208
Brand: Tocris Bioscience
SKU: 3565
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 3565

Citations (19)

COA / SDS

High affinity and selective EP4 antagonist

Key Product Details

Description:	High affinity and selective EP₄ antagonist
Chemical Name:	4-Cyano-2-[[2-(4-fluoro-1-naphthalenyl)-1-oxopropyl]amino]benzenebutanoic acid
Purity:	≥98% (HPLC)
Molecular Weight:	404.43

View Full Technical Details for ONO AE3 208

Technical Data
Citations

Biological Activity for ONO AE3 208

ONO AE3 208 is a high affinity and selective EP₄ receptor antagonist (K_i values are 1.3, 30, 790 and 2400 nM for EP₄, EP₃, FP and TP receptors respectively). Displays no affinity for EP₁, EP₂, DP or IP receptors (K_i >10 μM). Inhibits PGE₂-induced IL-8 production in colonic epithelial caco-2 cells and attenuates PGE₂ inhibition of natural killer T cell activation. Suppresses recovery from experimentally-induced colitis and stimulates CD4⁺ T cell proliferation in C57BL/6 mice. Also reduces metastasis of mammary tumor cells in a murine model of breast cancer. Orally active.

Technical Data for ONO AE3 208

M. Wt	404.43
Formula	C₂₄H₂₁FN₂O₃
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	402473-54-5
PubChem ID	10111831
InChi Key	MTDIMKNAJUQTIO-UHFFFAOYSA-N
Smiles	O=C(C(C)C2=C(C=CC=C3)C3=C(F)C=C2)NC1=CC(C#N)=CC=C1CCCC(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for ONO AE3 208

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	40.44	100

Preparing Stock Solutions for ONO AE3 208

The following data is based on the product molecular weight 404.43.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.47 mL	12.36 mL	24.73 mL
5 mM	0.49 mL	2.47 mL	4.95 mL
10 mM	0.25 mL	1.24 mL	2.47 mL
50 mM	0.05 mL	0.25 mL	0.49 mL

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References for ONO AE3 208

References are publications that support the biological activity of the product.

Deng Exosome-like nanoparticles from intestinal mucosal cells carry prostaglandin E2 and suppress activation of liver NKT cells. J.Immunol. 2013 PMID: 23467936
Dey Prostaglandin E₂ couples through EP₄ prostanoid receptors to induce IL-8 production in human colonic epithelial cell lines. Br.J.Pharmacol 2009 PMID: 19175605
Kabashima The prostaglandin receptor EP₄ suppresses colitis, mucosal damage and CD⁴ cell activation in the gut. J.Clin.Invest. 2002 PMID: 11927615
Ma Prostaglandin E receptor EP₄ antagonism inhibits breast cancer metastasis. Cancer Res. 2006 PMID: 16540639