NSC 109555 ditosylate is a selective, reversible, ATP-competitive Chk2 inhibitor (IC₅₀ = 0.2 μM) that displays no effect on a range of other kinases including Chk1 (IC₅₀ > 10 μM). Inhibits histone H1 phosphorylation (IC₅₀ = 0.24 μM) and attenuates mitochondrial ATP synthesis. Exhibits antiproliferative activity in a number of leukemias in vivo.

Product Specifications

Molecular Weight

752.86

Formula

C₁₉H₂₄N₁₀O.2C₇H₈O₃S

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

2,2'-[Carbonylbis(imino-4,1-phenyleneethylidyne]bishydrazinecarboximidamide) bis(4-methylbenzenesulfonate)

CAS Number

66748-43-4

PubChem ID

12449850

InChI Key

DIQGNVANOSOABS-XMDRLFCYSA-N

SMILES

O=C(NC2=CC=C(/C(C)=N/NC(N)=N)C=C2)NC1=CC=C(/C(C)=N/NC(N)=N)C=C1.CC3=CC=C(S(=O)(O)=O)C=C3.CC4=CC=C(S(=O)(O)=O)C=C4

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	7.53	10

Preparing Stock Solutions for NSC 109555 ditosylate

The following data is based on the product molecular weight 752.86

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	13.28 mL	66.41 mL	132.83 mL
0.5 mM	2.66 mL	13.28 mL	26.57 mL
1 mM	1.33 mL	6.64 mL	13.28 mL
5 mM	0.27 mL	1.33 mL	2.66 mL

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Background References

References are publications that support the biological activity of the product.

Byczkowski Potentiation of the antimitochondrial and antiproliferative effects of bis(guanylhydrazones) by phenethylbiguanide. Cancer Res. 1982 PMID: 6896674
Jobson Identification of a bis-guanylhydrazone [4,4'-diacetyldiphenylurea-bis(guanylhydrazone); NSC 109555] as a novel chemotype for inhibition of Chk2 kinase. Mol.Pharmacol. 2007 PMID: 17616632
Byczkowski Actions of bis(guanylhydrazones) on isolated rat liver mitochondria. Biochem.Pharmacol. 1981 PMID: 6895596

NSC 109555 ditosylate

Tocris Bioscience, part of Bio-Techne | Catalog # 3034

Key Product Details

Description

Alternative Names

View all Checkpoint Kinase Inhibitors »

Product Description