NS 3728

Name: NS 3728
Brand: Tocris Bioscience
SKU: 6973

Tocris Bioscience, part of Bio-Techne | Catalog # 6973

Citations (4)

COA / SDS

VRAC blocker

Key Product Details

Description:	VRAC blocker
Alternative Names:	Endovion
Chemical Name:	N-[3,5-bis(Trifluoromethyl)phenyl]-N'-[4-bromo-2-(2H-tetrazol-5-yl)phenyl]urea
Purity:	≥98% (HPLC)
Molecular Weight:	495.18

View Full Technical Details for NS 3728

Technical Data
Citations

Biological Activity for NS 3728

NS 3728 is a volume-regulated anion channel (VRAC) blocker (IC₅₀ = 460 nM). Blocks red blood cell chloride conductance (IC₅₀ = 0.6 μM). NS 3728 also inhibits Ca²⁺-activated Cl^- current (CaCC) by anoctamin (ANO) in an apparently voltage-dependent manner (IC₅₀ against ANO1 = 2.1 and 0.7 μM at -95 and +50 mV, respectively).

Technical Data for NS 3728

M. Wt	495.18
Formula	C₁₆H₉BrF₆N₆O
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	265646-85-3
PubChem ID	10005966
InChi Key	AEFYFGMSRKDXHZ-UHFFFAOYSA-N
Smiles	FC(F)(C1=CC(C(F)(F)F)=CC(NC(NC2=C(C3=NN=NN3)C=C(C=C2)Br)=O)=C1)F

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for NS 3728

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	49.52	100
	ethanol	24.76	50

Preparing Stock Solutions for NS 3728

The following data is based on the product molecular weight 495.18.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.02 mL	10.10 mL	20.19 mL
5 mM	0.40 mL	2.02 mL	4.04 mL
10 mM	0.20 mL	1.01 mL	2.02 mL
50 mM	0.04 mL	0.20 mL	0.40 mL

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Reconstitution Calculator

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References for NS 3728

References are publications that support the biological activity of the product.

Sauter ANO1 (TMEM16A) in pancreatic ductal adenocarcinoma (PDAC). Pflugers Arch. 2015 PMID: 25163766
Kjaer Chloride channel blockers inhibit iNOS expression and NO production in IFNgamma-stimulated microglial BV2 cells. Brain Res. 2009 PMID: 19446535
Godwin E-cadherin-deficient cells have synthetic lethal vulnerabilities in plasma membrane organisation, dynamics and function. Gastric Cancer 2019 PMID: 30066183
Helix Inhibition of the endogenous volume-regulated anion channel (VRAC) in HEK293 cells by acidic di-aryl-ureas. J.Membr.Biol. 2003 PMID: 14724745