NG 25

Name: NG 25
Brand: Tocris Bioscience
SKU: 5429
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 5429

(1)

Citations (4)

COA / SDS

TGF-β-activated kinase (TAK1) inhibitor

Key Product Details

Description:	TGF-β-activated kinase (TAK1) inhibitor
Chemical Name:	N-[4-[(4-Ethyl-1-piperazinyl)methyl]-3-(trifluoromethyl)phenyl]-4-methyl-3-(1H-pyrrolo[2,3-b]pyridin-4-yloxy)benzamide
Purity:	≥98% (HPLC)
Molecular Weight:	537.58

View Full Technical Details for NG 25

Technical Data
Citations / Reviews (1)

Biological Activity for NG 25

NG 25 is a TGF-β-activated kinase (TAK1) inhibitor (IC₅₀ = 149 nM). Also inhibits LYN, MAP4K2 and Abl kinase activity (IC₅₀ values are 12.9, 21.7 and 75.2 nM, respectively). Suppresses CpG A and CpG B-stimulated secretion of IFN-α and TLR7/8 agonist CL097-stimulated secretion of IFN-β in Gen 2.2 leukemia cells in vitro.

Licensing Information

Sold under license from Dana-Farber Cancer Institute.

Technical Data for NG 25

M. Wt	537.58
Formula	C₂₉H₃₀F₃N₅O₂
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1315355-93-1
PubChem ID	53340664
InChi Key	SMPGEBOIKULBCT-UHFFFAOYSA-N
Smiles	CCN1CCN(CC2=C(C(F)(F)F)C=C(NC(C3=CC(OC4=CC=NC5=C4C=CN5)=C(C)C=C3)=O)C=C2)CC1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for NG 25

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	10.75	20

Preparing Stock Solutions for NG 25

The following data is based on the product molecular weight 537.58.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	9.30 mL	46.50 mL	93.01 mL
1 mM	1.86 mL	9.30 mL	18.60 mL
2 mM	0.93 mL	4.65 mL	9.30 mL
10 mM	0.19 mL	0.93 mL	1.86 mL

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References for NG 25

References are publications that support the biological activity of the product.

Pauls Essential role for IKKβ in production of type 1 IF.s by plasmacytoid dendritic cells. J.Biol.Chem. 2012 PMID: 22511786
Dzamko The IkappaB kinase family phosphorylates the Parkinson's disease kinase LRRK2 at Ser935 and Ser910 during Toll-like receptor signaling. PLoS ONE 2012 PMID: 22723946
Tan Discovery of type II inhibitors of TGFβ-activated kinase 1 (TAK1) and mitogen-activated protein kinase kinase kinase kinase 2 (MAP4K2). J.Med.Chem. 2015 PMID: 25075558