N,N-Dimethylsphingosine

Product Description

N,N-Dimethylsphingosine is a competitive sphingosine kinase (SphK) inhibitor. Exhibits inhibitory activity in platelets and in cytosolic extracts from U937, Swiss 3T3 and PC12 cells. Selectively inhibits sphingosine phosphorylation; does not inhibit N-acylation, and displays no inhibitory effect on protein kinase C at concentrations that inhibit SphK. Increases the release of IL-1β and MCP-1 in cultured astrocytes; thought to mediate mechanical allodynia via this mechanism.

Solubility

Preparing Stock Solutions for N,N-Dimethylsphingosine

The following data is based on the product molecular weight 327.55

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.3 mM	10.18 mL	50.88 mL	101.77 mL
1.5 mM	2.04 mL	10.18 mL	20.35 mL
3 mM	1.02 mL	5.09 mL	10.18 mL
15 mM	0.20 mL	1.02 mL	2.04 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Edsall N,N-dimethylsphingosine is a potent competitive inhibitor of sphingosine kinase but not of protein kinase C: modulation of cellular levels of sphingosine 1-phosphate and ceramide. Biochemistry 1998 PMID: 9737868
• Yatomi N,N-dimethylsphingosine inhibition of sphingosine kinase and sphingosine 1-phosphate activity in human platelets. Biochemistry 1996 PMID: 8555236
• Patti Metabolomics implicates altered sphingolipids in chronic pain of neuropathic origin. Nat.Chem.Biol. 2012 PMID: 22267119