ML 162

Name: ML 162
Brand: Tocris Bioscience
SKU: 7821

Tocris Bioscience, part of Bio-Techne | Catalog # 7821

Citations (7)

COA / SDS

Inhibits glutathione peroxidase (GPX4); induces ferroptosis

Key Product Details

Description:	Inhibits glutathione peroxidase (GPX4); induces ferroptosis
Chemical Name:	2-[(Chloroacetyl)(3-chloro-4-methoxyphenyl)amino]-N-(2-phenylethyl)-2-thien-2-ylacetamide
Purity:	≥98% (HPLC)
Molecular Weight:	477.41

View Full Technical Details for ML 162

Technical Data
Citations

Biological Activity for ML 162

ML 162 is a covalent glutathione peroxidase 4 (GPX4) inhibitor. It induces ferroptosis in vivo. It is selectively lethal to mutant RAS oncogene-expressing cell lines (IC₅₀ = 25 and 578 nM for HRAS^G12V-expressing and wild-type BJ fibroblasts, respectively).

Technical Data for ML 162

M. Wt	477.41
Formula	C₂₃H₂₂Cl₂N₂O₃S
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1035072-16-2
PubChem ID	3689413
InChi Key	UNVKYJSNMVDZJE-UHFFFAOYSA-N
Smiles	O=C(NCCC=1C=CC=CC1)C(C=2SC=CC2)N(C(=O)CCl)C3=CC=C(OC)C(Cl)=C3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for ML 162

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	47.74	100
	ethanol	9.55	20

Preparing Stock Solutions for ML 162

The following data is based on the product molecular weight 477.41.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.09 mL	10.47 mL	20.95 mL
5 mM	0.42 mL	2.09 mL	4.19 mL
10 mM	0.21 mL	1.05 mL	2.09 mL
50 mM	0.04 mL	0.21 mL	0.42 mL

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References for ML 162

References are publications that support the biological activity of the product.

Karaj Tunable cysteine-targeting electrophilic heteroaromatic warheads induce ferroptosis. J.Med.Chem. 2022 PMID: 35984756
Weïwer Development of small-molecule probes that selectively kill cells induced to express mutant RAS. Bioorg.Med.Chem.Lett. 2012 PMID: 22297109
Luo Intracellular delivery of glutathione peroxidase degrader induces ferroptosis in vivo. Angew.Chem.Int.Ed.Engl. 2022 PMID: 35924720