Mitomycin C
Tocris Bioscience, part of Bio-Techne | Catalog # 3258
DNA cross-linking antitumor agent
Key Product Details
Description
DNA cross-linking antitumor agent
Alternative Names
Ametycine
Product Description
Mitomycin C is an antibiotic and antitumor agent. Covalently binds DNA forming intra- and interstrand crosslinks. Inhibits DNA synthesis. Also used for MEF/STO feeder layer preparation in stem cell culture.Product Specifications
Molecular Weight
334.33
Formula
C15H18N4O5
Storage
Store at +4°C
Purity
≥98% (HPLC)
Chemical Name
[1aS-(1aα,8β,8aα,8bα)]-6-Amino-8-[[(aminocarbonyl)oxy]methyl]-1,1a,2,8,8a,8b-hexahydro-8a-methoxy-5-methylazirinopyrrolo[1,2-a]indole-4,7-dione
CAS Number
50-07-7
PubChem ID
5746
InChI Key
NWIBSHFKIJFRCO-WUDYKRTCSA-N
SMILES
O=C(C(N)=C1C)C2=C(N(C[C@H]4[C@@H]3N4)[C@@]3(OC)[C@@H]2COC(N)=O)C1=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 0.33 | 1 | |
| DMSO | 33.43 | 100 |
Preparing Stock Solutions
for Mitomycin C
The following data is based on the product molecular weight 334.33
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.99 mL | 14.96 mL | 29.91 mL |
| 5 mM | 0.60 mL | 2.99 mL | 5.98 mL |
| 10 mM | 0.30 mL | 1.50 mL | 2.99 mL |
| 50 mM | 0.06 mL | 0.30 mL | 0.60 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Park Modeling and correction of structural variations in patient-derived iPSCs using CRISPR/Cas9. Nat.Protoc. 2016 PMID: 27711053
- Bizanek Isolation and structure of an intrastrand cross-link adduct of mito. C and DNA. Biochemistry 1992 PMID: 1554696
- Bryja Derivation of mouse embryonic stem cells. Nat.Protoc. 2006 PMID: 17487198
- Tomasz Isolation and structure of a covalent cross-link adduct between mito. C and DNA. Science 1987 PMID: 3103215
Product Specific Notices for Mitomycin C
For research use only
⚠ WARNING: This product can expose you to chemicals including Mitomycin C, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov
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