Lactacystin
Tocris Bioscience, part of Bio-Techne | Catalog # 2267
Cell-permeable, potent and selective proteasome inhibitor
Key Product Details
Description
Cell-permeable, potent and selective proteasome inhibitor
Product Description
Lactacystin is a cell-permeable, potent and selective proteasome inhibitor. A Streptomyces metabolite that is thought to bind irreversibly to the active site N-terminal threonine residue of the catalytic β-subunit of the 20S proteasome, thereby inhibiting its chymotrypsin and trypsin-like activities. Induces neurite outgrowth in Neuro 2a neuroblastoma cells and has been reported to induce apoptosis in human monoblast U937 cells. Also inhibits NF-κB activation (IC50 = 10 μM).Product Specifications
Molecular Weight
376.42
Formula
C15H24N2O7S
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
(2R,3S,4R)-3-Hydroxy-2-[(1S)-1-hydroxy-2-methylpropyl]-4-methyl-5-oxo-2-pyrrolidinecarboxy-N-acetyl-L-cysteine thioester
CAS Number
133343-34-7
PubChem ID
6610292
InChI Key
DAQAKHDKYAWHCG-RWTHQLGUSA-N
SMILES
O=C1N[C@]([C@@](SC[C@H](NC(C)=O)[C@](O)=O)=O)([C@H](C(C)C)O)[C@@H](O)[C@H]1C
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 3.76 | 10 |
Preparing Stock Solutions
for Lactacystin
The following data is based on the product molecular weight 376.42
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 26.57 mL | 132.83 mL | 265.66 mL |
| 0.5 mM | 5.31 mL | 26.57 mL | 53.13 mL |
| 1 mM | 2.66 mL | 13.28 mL | 26.57 mL |
| 5 mM | 0.53 mL | 2.66 mL | 5.31 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Fenteany Inhibition of proteasome activities and subunit-specific amino-terminal threonine modification by lactacystin. Science 1995 PMID: 7732382
- Bellas Inhibition of NF-κB activity induces apoptosis in murine hepatocytes. Am.J.Pathol. 1997 PMID: 9327720
- Omura Lactacystin, a novel microbial metabolite, induces neuritogenesis of neuroblastoma cells. J.Antibiot. 1991 PMID: 1848215
Product Specific Notices for Lactacystin
For research use only
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