L-Ascorbic acid

Product Description

L-Ascorbic acid is an inhibitor of Ca_v3.2 channels (IC₅₀ = 6.5 μM); displays no effect on Ca_v3.1 or Ca_v3.3 channels heterologously expressed in HEK 293 cells. Also enhances the generation of induced pluripotent stem cells (iPSCs) from mouse and human somatic cells by increasing reprogramming efficiency. Commonly used antifade reagent in live cell microscopy. Used for the generation of midbrain dopaminergic neurons from hPSCs or CiPSCs from MEFs (see protocols below). Naturally occurring antioxidant.

L-Ascorbic acid synthesized to Ancillary Material Grade also available.

For more information about how L-Ascorbic acid may be used, see our protocol: Highly Efficient Generation of CiPSCs from MEFs, Generating Midbrain Dopaminergic Neurons from hPSCs.

Solubility

Preparing Stock Solutions for L-Ascorbic acid

The following data is based on the product molecular weight 176.12

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

2 1 Recalculate

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
5 mM	1.14 mL	5.68 mL	11.36 mL
25 mM	0.23 mL	1.14 mL	2.27 mL
50 mM	0.11 mL	0.57 mL	1.14 mL
250 mM	0.02 mL	0.11 mL	0.23 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Cordes Mechanisms and advancement of antifading agents for fluorescence microscopy and single-molecule spectroscopy. Phys.Chem.Chem.Phys. 2011 PMID: 21311807
• Nelson Molecular mechanisms of subtype-specific inhibition of neuronal T-type calcium channels by ascorbate. J.Neurosci. 2007 PMID: 18003836
• Esteban Vitamin C enhances the generation of mouse and human induced pluripotent stem cells. Cell Stem Cell 2010 PMID: 20036631
• Padayatty Vitamin C as an antioxidant: evaluation of its role in disease prevention. J.Am.Coll.Nutr. 2003 PMID: 12569111