JW 74
Tocris Bioscience, part of Bio-Techne | Catalog # 5653
Wnt pathway inhibitor
Product Description
JW 74 is an inhibitor of canonical Wnt signaling (IC50 = 790 nM). Targets the β-catenin destruction complex (GSK-3 beta/AXIN/APC). Increases AXIN2 and decreases active β-catenin levels in colorectal cancer (CRC) cells. Also arrests cell cycle in G1 and inhibits proliferation of CRC cells in vitro. Inhibits tumor growth and formation in mouse models.Product Specifications
Molecular Weight
456.52
Formula
C24H20N6O2S
Storage
Store at +4°C
Purity
≥98% (HPLC)
Chemical Name
4-[4-(4-Methoxyphenyl)-5-[[[3-(4-methylphenyl)-1,2,4-oxadiazol-5-yl]methyl]thio]-4H-1,2,4-triazol-3-yl]-pyridine
CAS Number
863405-60-1
PubChem ID
16013152
InChI Key
KRIKILRRJCIWNB-UHFFFAOYSA-N
SMILES
COC1=CC=C(N2C(SCC3=NC(C4=CC=C(C)C=C4)=NO3)=NN=C2C5=CC=NC=C5)C=C1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 45.65 | 100 |
Preparing Stock Solutions
for JW 74
The following data is based on the product molecular weight 456.52
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.19 mL | 10.95 mL | 21.90 mL |
| 5 mM | 0.44 mL | 2.19 mL | 4.38 mL |
| 10 mM | 0.22 mL | 1.10 mL | 2.19 mL |
| 50 mM | 0.04 mL | 0.22 mL | 0.44 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Waaler Novel synthetic antagonists of canonical Wnt signaling inhibit colorectal cancer cell growth. Cancer Res. 2011 PMID: 21199802
Product Specific Notices for JW 74
For research use only
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