Can you provide the HPLC conditions for determining the purity of Imidazoquinoline Resiquimod, R-848?

Thank you for inquiry about the Imidazoquinoline Resiquimod, R-848, Cat No NBP2-26231. Each batch of NBP2-26231 is endotoxin free and equal to or greater than 98% pure by HPLC. Identity is determined by Mass Spec, 1H and 13C NMR .

We are interested in the Catalog NBP2-26231, R848. Is the R848 product water soluble and ready to use to stimulate some cell-line in vitro? Do we need another support product?

The R-848 Imidazoquinoline Resiquimod TLR7 and TLR8 ligand NBP2-26231 is provided solubilized in water at a concentration of 1.0mg/ml. The ligand is ready to use and does not require the purchase of any other support product.

Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand

Name: Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand
Brand: Novus Biologicals
SKU: NBP2-26231

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26231

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Product Datasheet / COA / SDS

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Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Functional Assay

Product Summary for Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand

CAS#: 144875-48-9.

Product Specifications

Application Notes

Identity determined by MS, 1H-NMR and 13C-NMR. This product is useful for activation of mouse TLR7 and human TLR7 and TLR8. Stimulation of TLR8 has been achieved with 0.5-5 ug/mL. Use in functional assays reported in scientific literature (PMID 26134251).

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25532693)

Scientific Data Images for Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand

Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand [NBP2-26231] - TLR8 stimulation with R-848 measured by SEAP Expression 293T cells were transfected with pCMV/TLR8 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 2 ug/mL or 5 ug/mL of R-848 was added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using NF-kB SEAPorterAssay kit.

Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand [NBP2-26231] - R-848 specifically activated the TLR7- or TLR8-depedent NF-kB/SEAP reporter cells in a dose dependent manner.

Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand [NBP2-26231] - Formula: C17H22N4O2

Formulation, Preparation, and Storage

Preparation Method

Dissolve in DMSO at a concentration of 10 mg/ml. If the substance is not fully soluble, it can be heated up to 60C to ensure complete solubilization. Also soluble in dichloromethane, 100% or 5% methanol; poorly soluble in acetonitrile or ethyl acetate.

Purification

>98%, by HPLC.

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20 °C.

Background: Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand

Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand is a low molecular weight synthetic molecule of the Imidazoquinoline family. It has potent immunostimulatory activity. It stimulates antibody secretion, cytokine production, protection from apoptosis and CD80 up-regulation. R-848 has been shown to activate only TLR7 and TLR8. This activation is MyD88 dependent and leads to the induction of the transcription factor NF-kB.

Alternate Names

4-Amino-2-(ethoxymethyl)-a,a-dimethyl-1H-imidazo[4,5-c]quinoline-1-ethanol, Imidazoquinoline Resiquimod, Imidazoquinoline Resiquimod (R-848),TLR7 & TLR8 ligand, Imidazoquinoline Resiquimod R-848, R-848, Resiquimod, S 28463

Additional Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand Products

Product Documents for Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand

Product Datasheets

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COA

SDS

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Product Specific Notices for Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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