GIT 27

Name: GIT 27
Brand: Tocris Bioscience
SKU: 3270

Tocris Bioscience, part of Bio-Techne | Catalog # 3270

Citations (14)

COA / SDS

Immunomodulator; reduces production of pro-inflammatory cytokines

Key Product Details

Description:	Immunomodulator; reduces production of pro-inflammatory cytokines
Chemical Name:	4,5-Dihydro-3-phenyl-5-isoxazoleacetic acid
Purity:	≥98% (HPLC)
Molecular Weight:	205.21

View Full Technical Details for GIT 27

Technical Data
Citations

Biological Activity for GIT 27

GIT 27 is an orally active immunomodulatory agent that primarily targets macrophages. Inhibits TNF-α secretion via interference of macrophage toll-like receptor (TLR) 4 and TLR 2/6 signaling pathway. Also reduces the secretion of pro-inflammatory cytokines IL1-β, IL-10 and IFN-γ. Antidiabetogenic; prevents IL-β and IFN-γ-induced pancreatic islet cell death in vitro.

Compound Libraries for GIT 27

GIT 27 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Antiviral Library. Find out more about compound libraries available from Tocris.

Technical Data for GIT 27

M. Wt	205.21
Formula	C₁₁H₁₁NO₃
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	6501-72-0
PubChem ID	10798271
InChi Key	MUFJHYRCIHHATF-UHFFFAOYSA-N
Smiles	O=C(CC1CC(C2=CC=CC=C2)=NO1)O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for GIT 27

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	20.52	100

Preparing Stock Solutions for GIT 27

The following data is based on the product molecular weight 205.21.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	4.87 mL	24.37 mL	48.73 mL
5 mM	0.97 mL	4.87 mL	9.75 mL
10 mM	0.49 mL	2.44 mL	4.87 mL
50 mM	0.10 mL	0.49 mL	0.97 mL

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References for GIT 27

References are publications that support the biological activity of the product.

Stojanovic In vitro, ex vivo and in vivo immunopharmacological activities of the isoxazoline compound VGX-1027: Modulation of cytokine synthesis and prevention of both organ-specific and systemic autoimmune diseases in murine models. Clin.Immunol. 2007 PMID: 17449326
Mangano In vitro inhibition of enterobacteria-reactive CD4+CD25- T cells and suppression of immunoinflammatory colitis in mice by the novel immunomodulatory agent VGX-1027. Eur.J.Pharmacol. 2008 PMID: 18374912
Stosic-Grujicic A potent immunomodulatory compound (S,R)-3-phenyl-4,5-dihydro-5-isoxasoleacetic acid, prevents spontaneous and accelerated forms of autoimmune diabetes in NOD mice and inhibits the immunoinflammatory diabetes induced by multiple low doses of stre J.Pharmacol.Exp.Ther. 2007 PMID: 17148780