Gemcitabine hydrochloride

Tocris Bioscience, part of Bio-Techne | Catalog # 3259

DNA synthesis inhibitor
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Key Product Details

Description

DNA synthesis inhibitor

View all DNA, RNA and Protein Synthesis Inhibitors »

Product Description

Gemcitabine hydrochloride is a deoxycytidine analog that inhibits DNA synthesis. Metabolized to form gemcitabine triphosphate (dFdCTP) and gemcitabine diphosphate (dFdCDP). dFdCTD inhibits ribonucleotide reductase causing a reduction in cellular nucleotides. dFdCTP is incorporated in DNA resulting in DNA strand termination. Displays antitumor activity in vitro and in vivo. Inhibits replication of MERS-CoV and SARS-CoV in vitro (EC50 values are 1.216 and 4.957 μM, respectively).

Product Specifications

Molecular Weight

299.66

Formula

C9H11F2N3O4.HCl

Storage

Desiccate at +4°C

Purity

≥98% (HPLC)

Chemical Name

(+)-2'-Deoxy-2',2'-difluorocytidine hydrochloride

CAS Number

122111-03-9

PubChem ID

60749

InChI Key

OKKDEIYWILRZIA-OSZBKLCCSA-N

SMILES

OC[C@@H]1[C@@H](O)C(F)(F)[C@H](N2C(N=C(N)C=C2)=O)O1.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 29.97 100
DMSO 5.99 20

Preparing Stock Solutions for Gemcitabine hydrochloride

The following data is based on the product molecular weight 299.66

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.34 mL 16.69 mL 33.37 mL
5 mM 0.67 mL 3.34 mL 6.67 mL
10 mM 0.33 mL 1.67 mL 3.34 mL
50 mM 0.07 mL 0.33 mL 0.67 mL

Calculators

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Background References

References are publications that support the biological activity of the product.

Product Documents for Gemcitabine hydrochloride

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Gemcitabine hydrochloride

For research use only

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