FK 866

Name: FK 866
Brand: Tocris Bioscience
SKU: 8072
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 8072

(1)

Citations (21)

COA / SDS

Potent and non-competitive NAMPT inhibitor; induces apoptosis and autophagy

Key Product Details

Description:	Potent and non-competitive NAMPT inhibitor; induces apoptosis and autophagy
Chemical Name:	(2E)-N-[4-(1-Benzoyl-4-piperidinyl)butyl]-3-(3-pyridinyl)-2-propenamide
Purity:	≥98% (HPLC)
Molecular Weight:	391.51

View Full Technical Details for FK 866

Technical Data
Citations / Reviews (1)

Biological Activity for FK 866

FK 866 is a non-competitive and high-affinity inhibitor of NAMPT (nicotinamide phosphoribosyltransferase, PBEF1) (K_i = 0.3 nM); inhibits NAD biosynthesis. Induces delayed cell death by apoptosis in HepG2 human liver carcinoma cells (IC₅₀ ~1 nM). Induces apoptosis in four different neuroblastoma cell lines; also induces autophagy in SH-SY5Y cells. Potentiates the cytotoxic effects induced by Etoposide (Cat. No. 1226) and Cisplatin (Cat. No. 2251).

Technical Data for FK 866

M. Wt	391.51
Formula	C₂₄H₂₉N₃O₂
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	658084-64-1
PubChem ID	6914657
InChi Key	KPBNHDGDUADAGP-VAWYXSNFSA-N
Smiles	C(=O)(N1CCC(CCCCNC(/C=C/C=2C=CC=NC2)=O)CC1)C3=CC=CC=C3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for FK 866

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	39.15	100
	ethanol	39.15	100

Preparing Stock Solutions for FK 866

The following data is based on the product molecular weight 391.51.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.55 mL	12.77 mL	25.54 mL
5 mM	0.51 mL	2.55 mL	5.11 mL
10 mM	0.26 mL	1.28 mL	2.55 mL
50 mM	0.05 mL	0.26 mL	0.51 mL

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References for FK 866

References are publications that support the biological activity of the product.

Travelli Reciprocal potentiation of the antitumoral activities of FK866, an inhibitor of nicotinamide phosphoribosyltransferase, and etop. or cisp. in neuroblastoma cells. J.Pharmacol.Exp.Ther. 2011 PMID: 21685314
Galli Synthesis and biological evaluation of isosteric analogues of FK866, an inhibitor of NAD salvage. Chem.Med.Chem. 2008 PMID: 18247435
Hasmann FK866, a highly specific noncompetitive inhibitor of nicotinamide phosphoribosyltransferase, represents a novel mechanism for induction of tumor cell apoptosis. Cancer Res. 2003 PMID: 14612543