Filipin III

Tocris Bioscience, part of Bio-Techne | Catalog # 6250

Fluorescent cholesterol stain, also polyene antibiotic. Used for: Fluorescent cholesterol stain. Binds sterol in fungal membranes. Application: confocal microscopy
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Key Product Details

Description

Fluorescent cholesterol stain, also polyene antibiotic. Used for: Fluorescent cholesterol stain. Binds sterol in fungal membranes. Application: confocal microscopy

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Product Description

Key information: filipin III is a fluorescent cholesterol stain; polyene antibiotic.

Used for: fluorescent cholesterol staining. Binds sterols in fungal membranes. Also inhibits formation of the pathological form of prion protein (PrP-res) from the normal membrane-bound (PrP-sen) form.

Application: confocal microscopy.

Properties and Photophysical Data: excitation and emission maxima (λ) are 405 nm and 420-480 nm, respectively.

Product Specifications

Molecular Weight

654.83

Formula

C35H58O11

Storage

Store at -20°C

Purity

≥90% (HPLC)

Chemical Name

(3R,4S,6S,8S,10R,12R,14R,16S,17E,19E,21E,23E,25E,27S,28R)-4,6,8,10,12,14,16,27-Octahydroxy-3-[(1R)-1-hydroxyhexyl]-17,28-dimethyloxacyclooctacosa-17,19,21,23,25-pentaen-2-one

CAS Number

480-49-9

PubChem ID

6433553

InChI Key

IMQSIXYSKPIGPD-ZPXVTHDFSA-N

SMILES

C[C@@H](O1)[C@@H](O)/C=C/C=C/C=C/C=C/C=C(C)/[C@@H](O)C[C@H](O)C[C@H](O)C[C@H](O)C[C@H](O)C[C@H](O)C[C@H](O)[C@]([C@H](O)CCCCC)([H])C1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 3.27 5

Preparing Stock Solutions for Filipin III

The following data is based on the product molecular weight 654.83

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.05 mM 30.54 mL 152.71 mL 305.42 mL
0.25 mM 6.11 mL 30.54 mL 61.08 mL
0.5 mM 3.05 mL 15.27 mL 30.54 mL
2.5 mM 0.61 mL 3.05 mL 6.11 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for Filipin III

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Filipin III

For research use only

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