Ethyl LipotF

Name: Ethyl LipotF
Brand: Tocris Bioscience
SKU: 5950

Tocris Bioscience, part of Bio-Techne | Catalog # 5950

COA / SDS

Selective FTO inhibitor

Key Product Details

Description:	Selective FTO inhibitor
Chemical Name:	Ethyl (E)-4-(2-(4-benzylnicotinoyl)hydrazinyl)-4-oxobut-2-enoate
Purity:	≥98% (HPLC)
Molecular Weight:	353.38

View Full Technical Details for Ethyl LipotF

Technical Data
Citations

Biological Activity for Ethyl LipotF

Ethyl LipotF is a selective FTO inhibitor (IC₅₀ = 0.81 μM). Exhibits >30-fold selectivity for FTO over ALKBH2, 3 and 5 and >100-fold selectivity over PHD2 and JMJD2A. Increases levels of modified N⁶-methyladenosine (m6A) in HeLa cells in a concentration-dependent manner.

Technical Data for Ethyl LipotF

M. Wt	353.38
Formula	C₁₉H₁₉N₃O₄
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1808089-27-1
InChi Key	XUKLGMSFWAEOPR-CMDGGOBGSA-N
Smiles	O=C(NNC(/C=C/C(OCC)=O)=O)C1=C(CC2=CC=CC=C2)C=CN=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Ethyl LipotF

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	ethanol	7.07	20
	DMSO	35.34	100

Preparing Stock Solutions for Ethyl LipotF

The following data is based on the product molecular weight 353.38.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.83 mL	14.15 mL	28.30 mL
5 mM	0.57 mL	2.83 mL	5.66 mL
10 mM	0.28 mL	1.41 mL	2.83 mL
50 mM	0.06 mL	0.28 mL	0.57 mL

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Reconstitution Calculator

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References for Ethyl LipotF

References are publications that support the biological activity of the product.

Cheong A fluorescent methylation-switchable probe for highly sensitive analysis of FTO N⁶-methyladenosine demethylase activity in cells. Chem.Sci. 2018 PMID: 30288236
Toh A strategy based on nucleotide specificity leads to a subfamily-selective and cell-active inhibitor of N⁶-methyladenosine demethylase FTO. Chem.Sci. 2015 PMID: 28553460