EN6

Name: EN6
Brand: Tocris Bioscience
SKU: 7474

Tocris Bioscience, part of Bio-Techne | Catalog # 7474

COA / SDS

H+-ATPase activator; activates autophagy; inactivates mTORC1 signaling

Key Product Details

Description:	H⁺-ATPase activator; activates autophagy; inactivates mTORC1 signaling
Chemical Name:	N-[4-Fluoro-3-[(1-oxo-2-propen-1-yl)amino]phenyl]-1-(2-fluorophenyl)-1H-pyrazole-4-carboxamide
Purity:	≥98% (HPLC)
Molecular Weight:	368.34

View Full Technical Details for EN6

Technical Data
Citations

Biological Activity for EN6

EN6 is an activator of lysosomal vacuolar H⁺ ATPase (v-ATPase). EN6 covalently binds to the catalytic subunit of v-ATPase leading to increased acidification of lysosomes, inactivation of mTORC1 signaling and activation of autophagy in vivo. EN6 triggers entosis and promotes clearance of TDP43 protein aggregates in vitro.

Technical Data for EN6

M. Wt	368.34
Formula	C₁₉H₁₄F₂N₄O₂
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1808714-73-9
PubChem ID	99640033
InChi Key	SUSXQEYPNDORDQ-UHFFFAOYSA-N
Smiles	O=C(C=C)NC1=CC(=CC=C1F)NC(=O)C=2C=NN(C2)C=3C=CC=CC3F

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for EN6

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	3.68	10

Preparing Stock Solutions for EN6

The following data is based on the product molecular weight 368.34.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	27.15 mL	135.74 mL	271.49 mL
0.5 mM	5.43 mL	27.15 mL	54.30 mL
1 mM	2.71 mL	13.57 mL	27.15 mL
5 mM	0.54 mL	2.71 mL	5.43 mL

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References for EN6

References are publications that support the biological activity of the product.

Su Role and dynamics of vacuolar pH during cell-in-cell mediated death. Cell Death Dis. 2021 PMID: 33483474
Chung Covalent targeting of the vacuolar H⁺-ATPase activates autophagy via mTORC1 inhibition. Nat.Chem.Biol. 2019 PMID: 31285595

EN6