EHNA hydrochloride

Name: EHNA hydrochloride
Brand: Tocris Bioscience
SKU: 1261

Tocris Bioscience, part of Bio-Techne | Catalog # 1261

Citations (39)

COA / SDS

Adenosine deaminase inhibitor

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Technical Data
Citations

Product Description
Product Specifications
Solubility
Preparing Stock Solutions
Calculators
Background References
Product Documents
Specific Notices

Key Product Details

Description

Adenosine deaminase inhibitor

View all Deaminase Inhibitors »

Product Description

EHNA hydrochloride is a selective inhibitor of cGMP-stimulated phosphodiesterase (PDE2) (IC₅₀ = 0.8 - 4 μM). Also a potent inhibitor of adenosine deaminase. Suppresses spontaneous differentiation of human ESCs in feeder-free conditions. Also prevents directed neuronal differentiation.

Product Specifications

Molecular Weight

313.83

Formula

C₁₄H₂₃N₅O.HCl

Storage

Store at RT

Purity

≥98% (HPLC)

Chemical Name

erythro-9-(2-Hydroxy-3-nonyl)adenine hydrochloride

CAS Number

58337-38-5

PubChem ID

11056106

InChI Key

VVDXNJRUNJMYOZ-DHXVBOOMSA-N

SMILES

NC1=NC=NC2=C1N=CN2[C@@H](CCCCCC)[C@H](O)C.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	31.38	100
	DMSO	31.38	100
	Ethanol	31.38	100

Preparing Stock Solutions for EHNA hydrochloride

The following data is based on the product molecular weight 313.83

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.19 mL	15.93 mL	31.86 mL
5 mM	0.64 mL	3.19 mL	6.37 mL
10 mM	0.32 mL	1.59 mL	3.19 mL
50 mM	0.06 mL	0.32 mL	0.64 mL

Calculators

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

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Background References

References are publications that support the biological activity of the product.

Michie Rapid regulation of PDE-2 and PDE-4 cyclic AMP phosphodiesterase activity following ligation of the T-cell antigen receptor on thymocytes: analysis using the selective inhibitors erythro-9-(2-hydroxy-3-nonyl)-adenine (EHNA) and rolipram. Cell.Signal. 1996 PMID: 8730511
Podzuweit Isozyme selective inhibition of cGMP-stimulated cyclic nucleotide phosphodiesterases by erythro-9-(2-hydroxy-3-nonyl)adenine. Cell.Signal. 1995 PMID: 8519602
Bessodes Effect of chirality in erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA) on adenosine deaminase inhibition. Biochem.Pharmacol. 1982 PMID: 7082355