E 64
Tocris Bioscience, part of Bio-Techne | Catalog # 5208
Potent and irreversible cysteine protease inhibitor
Key Product Details
Description
Potent and irreversible cysteine protease inhibitor
Product Description
E 64 is a potent and irreversible cysteine protease inhibitor (IC50 values are 1.4, 2.5 and 4.1 nM for cathepsin K, L and S respectively. Also inhibits papain, calpain, cathepsin B and cathepsin H. Has no effect on serine proteases. Reduces HSC-3 tongue cancer cell invasion and induces oxidative stress and apoptosis in filarial parasites. Also inhibits autophagy-associated lysosomal degradation in vitro.Product Specifications
Molecular Weight
357.41
Formula
C15H27N5O5
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
(2S,3S)-3-[[[(1S)-1-[[[4-(Aminoiminomethyl)amino]butyl]amino]carbonyl]-3-methylbutyl]amino]carbonyl]-2-oxiranecarboxylic acid
CAS Number
66701-25-5
PubChem ID
123985
InChI Key
LTLYEAJONXGNFG-DCAQKATOSA-N
SMILES
O=[C@@](N[C@H]([C@](NCCCCNC(N)=N)=O)CC(C)C)[C@@H]1[C@@H](C(O)=O)O1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 7.15 | 20 |
Preparing Stock Solutions
for E 64
The following data is based on the product molecular weight 357.41
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 13.99 mL | 69.95 mL | 139.90 mL |
| 1 mM | 2.80 mL | 13.99 mL | 27.98 mL |
| 2 mM | 1.40 mL | 6.99 mL | 13.99 mL |
| 10 mM | 0.28 mL | 1.40 mL | 2.80 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Zhao Cytosolic FoxO1 is essential for the induction of autophagy and tumour suppressor activity. Nat.Cell.Biol. 2010 PMID: 20543840
- Bitu Cathepsin K is present in invasive oral tongue squamous cell carcinoma in vivo and in vitro. PLoS ONE 2013 PMID: 23951042
- Wadhawan Inhibition of cathepsin B by E-64 induces oxidative stress and apoptosis in filarial parasite. PLoS ONE 2014 PMID: 24667798
- Susa Human primary osteoclasts: in vitro generation and applications as pharmacological and clinical assay. J.Transl.Med. 2004 PMID: 15025786
Product Specific Notices for E 64
For research use only
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