dTAG-47
Tocris Bioscience, part of Bio-Techne | Catalog # 7530
Degrades mutant FKBP12F36V fusion proteins; useful alternative to genetic methods for target validation
Key Product Details
Description
Degrades mutant FKBP12F36V fusion proteins; useful alternative to genetic methods for target validation
Product Description
dTAG-47 is a degrader targeting mutant FKBP12F36V fusion proteins. Comprises a ligand selective for F36V single-point mutated FKBP12, a linker and a cereblon-binding ligand. Application of dTAG-47 induces rapid, reversible and selective degradation of FKBP12F36V fusion proteins in vitro and in vivo. dTAG is generalizable to a range of fusion proteins; useful as an alternative to genetic methods for target validation. See also dTAG-13 and dTAG-7.Negative control (Cat. No. 7531) also available.
FKBP12F36V can be expressed as a fusion with a target protein of interest using genome engineering techniques, via transgene expression or CRISPR-mediated locus-specific knock-in.
Plasmid vectors for the lentiviral expression and CRISPR-mediated knock-in of FKBP12F36V are available from Addgene.
Licensing Information
Sold under license from Dana-Farber Cancer InstituteProduct Specifications
Molecular Weight
1076.25
Formula
C59H73N5O14
Storage
Store at -20°C
Purity
≥97% (HPLC)
Chemical Name
(1R)-3-(3,4-Dimethoxyphenyl)-1-(2-(2-((8-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-5-yl)amino)octyl)amino)-2-oxoethoxy)phenyl)propyl (2S)-1-((S)-2-(3,4,5-trimethoxyphenyl)butanoyl)piperidine-2-carboxylate
CAS Number
2265886-81-3
PubChem ID
153280948
InChI Key
OQXAQHQPOQLCAE-ODPQSFPTSA-N
SMILES
CC[C@@H](C1=CC(OC)=C(C(OC)=C1)OC)C(N2CCCC[C@H]2C(O[C@@H](C3=C(C=CC=C3)OCC(NCCCCCCCCNC4=CC=C5C(N(C(C5=C4)=O)C6CCC(NC6=O)=O)=O)=O)CCC7=CC(OC)=C(C=C7)OC)=O)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 107.62 | 100 |
Preparing Stock Solutions
for dTAG-47
The following data is based on the product molecular weight 1076.25
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 0.93 mL | 4.65 mL | 9.29 mL |
| 5 mM | 0.19 mL | 0.93 mL | 1.86 mL |
| 10 mM | 0.09 mL | 0.46 mL | 0.93 mL |
| 50 mM | 0.02 mL | 0.09 mL | 0.19 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Layden A protocol for rapid degradation of endogenous transcription factors in mammalian cells and identification of direct regulatory targets. STAR Protoc. 2021 PMID: 34041503
- Weintraub YY1 is a structural regulator of enhancer-promoter loops. Cell 2017 PMID: 29224777
- Brunetti Mutant NPM1 maintains the leukemic state through HOX expression. Cancer Cell. 2018 PMID: 30205049
- Sreekanth Chemogenetic system demonstrates that Cas9 longevity impacts genome editing outcomes. ACS Cent.Sci. 2020 PMID: 33376784
- Bryan WDR5 is a conserved regulator of protein synthesis gene expression. Nucleic Acids Res. 2020 PMID: 31996893
- Popay MYC regulates ribosome biogenesis and mitochondrial gene expression programs through its interaction with host cell factor-1. Elife 2021 PMID: 33416496
Product Specific Notices for dTAG-47
For research use only
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