Dooku 1

Name: Dooku 1
Brand: Tocris Bioscience
SKU: 6568

Tocris Bioscience, part of Bio-Techne | Catalog # 6568

Citations (18)

COA / SDS

Reversibly blocks Yoda1 activity; does not affect constitutive Piezo1 activity

Key Product Details

Description:	Reversibly blocks Yoda1 activity; does not affect constitutive Piezo1 activity
Chemical Name:	2-[(2,6-Dichlorobenzyl)thio)-5-(1H-pyrrol-2-yl)-1,3,4-oxadiazole
Purity:	≥98% (HPLC)
Molecular Weight:	326.2

View Full Technical Details for Dooku 1

Technical Data
Citations

Biological Activity for Dooku 1

Dooku 1 reversibly blocks Yoda1 (Cat. No. 5586) activity (IC₅₀ = ~1.5 μM). Exhibits no effect on constitutive Piezo1 channel activity, but antagonizes Yoda1-induced Piezo1 activation in endothelial cells, and inhibits Yoda1-induced Ca²⁺-entry in vitro. Also blocks Yoda1-induced relaxation of aorta ex vivo.

Technical Data for Dooku 1

M. Wt	326.2
Formula	C₁₃H₉Cl₂N₃OS
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	2253744-54-4
PubChem ID	137321150
InChi Key	MNPOBXLPCWFONX-UHFFFAOYSA-N
Smiles	ClC1=C(CSC2=NN=C(O2)C3=CC=CN3)C(Cl)=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Dooku 1

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	32.62	100

Preparing Stock Solutions for Dooku 1

The following data is based on the product molecular weight 326.2.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.07 mL	15.33 mL	30.66 mL
5 mM	0.61 mL	3.07 mL	6.13 mL
10 mM	0.31 mL	1.53 mL	3.07 mL
50 mM	0.06 mL	0.31 mL	0.61 mL

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References for Dooku 1

References are publications that support the biological activity of the product.

Evans Yoda1 analogue (Dooku1) which antagonizes Yoda1-evoked activation of Piezo1 and aortic relaxation. Br.J.Pharmacol. 2018 PMID: 29498036