Digoxigenin NHS ester
Tocris Bioscience, part of Bio-Techne | Catalog # 7784
NHS ester hapten for labeling amino groups
Key Product Details
Description
NHS ester hapten for labeling amino groups
Product Description
Digoxigenin NHS ester is an NHS ester hapten for labeling amino groups within biomolecules (oligonucleotides or proteins). Digoxigenin NHS ester is also used for in situ hybridization.Product Specifications
Molecular Weight
658.79
Formula
C35H50N2O10
Storage
Store at -20°C
Purity
≥80% (HPLC)
Chemical Name
(3β,5β,12β)-3-[2-[[6-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]amino]-2-oxoethoxy]-12,14-dihydroxycard-20(22)-enolide
CAS Number
129273-26-3
InChI Key
KHNDABJZSPPYLE-FUGFVFQCSA-N
SMILES
O[C@@]12[C@@]([C@@H](C(CO3)=CC3=O)CC1)([C@@H](C[C@@]4([H])[C@@]2([H])CC[C@@]5([H])[C@@]4(CC[C@@H](C5)OCC(NCCCCCC(ON6C(CCC6=O)=O)=O)=O)C)O)C
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMF | 65.88 | 100 | |
| DMSO | 65.88 | 100 |
Preparing Stock Solutions
for Digoxigenin NHS ester
The following data is based on the product molecular weight 658.79
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.52 mL | 7.59 mL | 15.18 mL |
| 5 mM | 0.30 mL | 1.52 mL | 3.04 mL |
| 10 mM | 0.15 mL | 0.76 mL | 1.52 mL |
| 50 mM | 0.03 mL | 0.15 mL | 0.30 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Heyduk Peptide ligand-based ELISA reagents for antibody detection. Anal.Biochem. 2018 PMID: 30130491
Product Specific Notices for Digoxigenin NHS ester
For research use only
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