Dibutyryl-cAMP, sodium salt

Name: Dibutyryl-cAMP, sodium salt
Brand: Tocris Bioscience
SKU: 1141

Tocris Bioscience, part of Bio-Techne | Catalog # 1141

Citations (51)

COA / SDS

Cell-permeable cAMP analog

Key Product Details

Description:	Cell-permeable cAMP analog
Alternative Names:	Bucladesine sodium
Chemical Name:	N⁶,O^2'-Dibutyryladenosine 3',5'-cyclic monophosphate sodium salt
Purity:	≥98% (HPLC)
Molecular Weight:	491.37

View Full Technical Details for Dibutyryl-cAMP, sodium salt

Technical Data
Citations

Biological Activity for Dibutyryl-cAMP, sodium salt

Dibutyryl-cAMP sodium salt is a cell-permeable analog of cAMP that activates cAMP-dependent protein kinases and is a phosphodiesterase inhibitor. Combined with other reagents, dibutyryl-cAMP promotes differentiation of neural stem/progenitor cells (NSPCs) and other cell lines (see our protocol below) and increases survival rates of NSPCs and differentiation into neurons in vivo. Promotes neurite outgrowth in cell cultures. Dibutyryl-cAMP has anti-inflammatory activity and is used to promote wound healing. Choline acetyltransferase and vesicular acetylcholine transporter mRNA are increased in cells treated with dibutyryl-cAMP.

For more information about how Dibutyryl-cAMP, sodium salt may be used, see our protocol: Generating Midbrain Dopaminergic Neurons from hPSCs

Compound Libraries for Dibutyryl-cAMP, sodium salt

Dibutyryl-cAMP, sodium salt is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for Dibutyryl-cAMP, sodium salt

M. Wt	491.37
Formula	C₁₈H₂₃N₅NaO₈P
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	16980-89-5
PubChem ID	23663967
InChi Key	KRBZRVBLIUDQNG-JBVYASIDSA-M
Smiles	[Na+].[H][C@@]12COP([O-])(=O)O[C@@]1([H])[C@@H](OC(=O)CCC)[C@@H](O2)N1C=NC2=C1N=CN=C2NC(=O)CCC

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Dibutyryl-cAMP, sodium salt

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	49.14	100
	DMSO	49.14	100

Preparing Stock Solutions for Dibutyryl-cAMP, sodium salt

The following data is based on the product molecular weight 491.37.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.04 mL	10.18 mL	20.35 mL
5 mM	0.41 mL	2.04 mL	4.07 mL
10 mM	0.20 mL	1.02 mL	2.04 mL
50 mM	0.04 mL	0.20 mL	0.41 mL

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References for Dibutyryl-cAMP, sodium salt

References are publications that support the biological activity of the product.

Kim Effects of dibutyryl cyclic-AMP on survival and neuronal differentiation of neural stem/progenitor cells transplanted into spinal cord injured rats. PLoS One 2011 PMID: 21738784
Xia Transcriptional comparison of human induced and primary midbrain DArgic neurons. Sci.Rep. 2016 PMID: 26842779
Hei Lack of correlation between activation of cyclic AMP-dependent protein kinase and inhibition of contraction of rat vas deferens by cyclic AMP analogs Mol.Pharmacol. 1991 PMID: 1847496
Imamura Differential expression of dystrophin isoforms and utrophin during dibutyryl-cAMP-induced morphological differentiation of rat brain astrocytes. Proc.Natl.Acad.Sci.U.S.A. 1998 PMID: 9600931
Carranza Protein kinase A induces recruitment of active Na⁺,K⁺-ATPase units to the plasma membrane of rat proximal convoluted tubule cells. J.Physiol. 1998 PMID: 9679177