Cyclosporin A is an immunosuppressant; simultaneously binds calcineurin and cyclophilin and inhibits phosphatase activity of calcineurin (IC₅₀ = 5 nM). Additionally inhibits formation and opening of the mitochondrial permeability transition pore (MPTP). Also inhibits coronavirus replication in vitro. Enhances lentirival transduction of bone marrow-derived CD34⁺ cells and is additive with Prostaglandin E₂ (Cat. No. 2296).

Product Specifications

Molecular Weight

1202.63

Formula

C₆₂H₁₁₁N₁₁O₁₂

Storage

Store at -20°C

Purity

≥98% (HPLC)

CAS Number

59865-13-3

PubChem ID

2909

InChI Key

PMATZTZNYRCHOR-CGLBZJNRSA-N

SMILES

[H][C@@]1(C(C)C)NC([C@@H](N(C(CN(C([C@@H](NC([C@]([C@@H]([C@@H](C/C=C/C)C)O)(N(C([C@](C(C)C)(N(C([C@@H](N(C([C@](CC(C)C)(N(C([C@H](NC([C@@H](NC([C@@H](N(C1=O)C)CC(C)C)=O)C)=O)C)=O)C)[H])=O)C)CC(C)C)=O)C)[H])=O)C)[H])=O)CC)=O)C)=O)C)CC(C)C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	120.26	100
	Ethanol	60.13	50

Preparing Stock Solutions for Cyclosporin A

The following data is based on the product molecular weight 1202.63

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	0.83 mL	4.16 mL	8.32 mL
5 mM	0.17 mL	0.83 mL	1.66 mL
10 mM	0.08 mL	0.42 mL	0.83 mL
50 mM	0.02 mL	0.08 mL	0.17 mL

Calculators

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

Background References

References are publications that support the biological activity of the product.

Carbajo-Lozoya Human coronavirus NL63 replication is cyclophilin A-dependent and inhibited by non-immunosuppressive cyclosporine A-derivatives including Alisporivir. Virus Res. 2014 PMID: 24566223
de Wilde Cyclosporin A inhibits the replication of diverse coronaviruses. J.Gen.Virol. 2011 PMID: 21752960
de Wilde MERS-coronavirus replication induces severe in vitro cytopathology and is strongly inhibited by cyclosporin A or interferon-α treatment. J.Gen.Virol. 2013 PMID: 23620378
Petrillo Assessing the impact of cyclosporin A on lentiviral transduction and preservation of human hematopoietic stem cells in clinically relevant ex vivo gene therapy settings. Hum.Gene.Ther. 2019 PMID: 31037976
Yan Cyclosporin-A potently induces highly cardiogenic progenitors from embryonic stem cells. Biochem.Biophys.Res.Commun. 2009 PMID: 19094963
Nakata Evidence that cyclosporin A and dexameth. inhibit allergic airway inflammation via suppression of interleukin-5 synthesis by T cells. Br.J.Pharmacol. 1998 PMID: 9723954
Fruman Calcineurin phosphatase activity in T lymphocytes is inhibited by FK 506 and cyclosporin A. Proc.Natl.Acad.Sci.U.S.A. 1992 PMID: 1373887
Brookemeier and Pfeiffer Inhibition of the mitochondrial permeability transition pore by cyclosporin A during long time frame experiments: relationship between pore opening and the activity of mitochondrial phospholipases. Biochemistry 1995 PMID: 8845372

Cyclosporin A

Tocris Bioscience, part of Bio-Techne | Catalog # 1101

Key Product Details

Description

Alternative Names

View all Protein Ser/Thr Phosphatase Inhibitors »

Product Description