CMP-Azido-Sialic Acid
R&D Systems, part of Bio-Techne | Catalog # ES102
Activated Sugar
Key Product Details
Key Benefits
Learn more about Fluorescent Glycan Labeling and Detection
Species
Multi-Species
Conjugate
Azido
Product Summary for CMP-Azido-Sialic Acid
|
Formula |
C20H30N7O15P |
|
Molecular Weight |
639.46 Da |
|
Formulation |
1 mM provided in 20 mM Tris, pH 8.0 |
|
Stability & Storage |
Store the unopened product at < -20 °C. Good for 12 months from date of receipt. |
Incorporate or detect sialic acid without expensive specialized equipment!
Applications
- For in vitro enzymatic incorporation of azido-sugars into specific, targeted glycans.
- Detecting the presence or absence of sialated glycans.
- Labeling terminal sialoglycans on proteins and lipids.
Key Features and Benefits
- Can detect terminal sialoglycans without specialized, expensive equipment.
- Can be introduced to proteins and lipids via various sialyltransferases.
- Can be conjugated to desired reporter molecules, such as biotin, via click chemistry.
- Can be detected via Western blot, ELISA, and flow cytometry, depending on the type of reporter molecule.
- Has minimal side effects on target molecules.
- User-friendly.
For Details: Wu et al., (2015) Carbohydrate Res. 412:1-6
Related Reagents
Click Chemistry
Enzymes and Detection Reagents for CMP-Sialic-Acid, ES102
|
Sialyltransferases |
Sialation Link Specificity |
|---|---|
| ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 | Adds Sialic Acid to Galactose |
| ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 | Adds Sialic Acid to Galactose |
| ST3GAL5/GM3 Synthase | Adds Sialic Acid to Galactose |
| ST6 Gal Sialyltransferase 1/ST6GAL1 | Adds Sialic Acid to Galactose |
| ST6 Sialyltransferase 2/ST6GALNAC2 | Adds Sialic Acid to GalNAc |
| ST6GALNAC4 | Adds Sialic Acid to GalNAc |
| ST6 GalNAc alpha-2,6-sialyltransferaseV/ST6GALNAC5 | Adds Sialic Acid to GalNAc |
| ST6 Sialyltransferase 6/ST6GALNAC6 | Adds Sialic Acid to GalNAc |
| ST6 Gal Sialyltransferase 2/ST6GAL2 | Adds Sialic Acid to Galactose |
| ST6 Sialyltransferase 1/ST6GALNAC1 | Adds Sialic Acid to GalNAc |
| ST8 alpha-2,8-Sialyltransferase 8A/ST8SIA1 | Adds Sialic Acid to Sialic Acid |
| ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 | Adds Sialic Acid to Sialic Acid |
| ST8 alpha-2,8-Sialyltransferase 4/ST8SIA4 | Adds Sialic Acid to Sialic Acid |
- Viral Neuraminidase
- Bacterial Neuraminidase
- Streptavidin-HRP
Schematic
|
Terminal sialic acid can be detected on O- or N-linked glycans. Sialic acid is removed using a Sialidase. The specificity of Sialyltransferases allows you to add Azido-Sialic Acid to open positions. Azido-Sialic Acid can be detected using Biotinylated Alkyne in a click chemistry reaction. |
Data Examples
|
Labeling and detection of sialic acid on O-glycans on Bovine Fetuin. Fetuin (left side of the molecular marker; MW) and Desialylated Fetuin (right side of the molecular marker) were incubated with increasing amount of rhST3GAL1 (5, 25, 50, 250 ng) and 0.5 nmol CMP-Azido-Sialic Acid. Fetuin was treated with rcpNeuraminidase to prepare desialylated Fetuin. The reactions were then conjugated with Biotinylated Alkyne and separated by SDS-PAGE. The separated proteins were then blotted to a nitrocellulose membrane and detected with conventional Streptavidin-HRP chemiluminescence reagents. A. Total protein staining. B. Streptavidin-HRP detection of rhST3GAL1-mediated CMP-Azido-Sialic Acid incorporation. No incorporation of CMP-Azido-Sialic Acid is detected in fully sialted Fetuin. In contrast, strong labeling indicates CMP-Azido-Sialic Acid is incorporated into desialated Fetuin. Data adapted from Wu et al., (2015) Carbohydrate Res. 412:1-6. |
Formulation, Preparation, and Storage
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Product Documents for CMP-Azido-Sialic Acid
Product Specific Notices for CMP-Azido-Sialic Acid
For research use only
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