Clozapine N-oxide dihydrochloride
Tocris Bioscience, part of Bio-Techne | Catalog # 6329
Activator of hM3Dq and hM4Di DREADDs; water soluble version of Clozapine N-oxide (Cat. No. 4936)
Key Product Details
Description
Activator of hM3Dq and hM4Di DREADDs; water soluble version of Clozapine N-oxide (Cat. No. 4936)
Alternative Names
CNO (water-soluble)
Product Description
Clozapine N-oxide dihydrochloride is a water-soluble salt of Clozapine N-oxide (Cat. No.4936). Activator of muscarinic DREADDs. Clozapine N-oxide dihydrochloride exhibits improved bioavailability (6 - 7-fold higher plasma concentration) compared with CNO, with less conversion to clozapine in animal studies.Product Specifications
Molecular Weight
415.74
Formula
C18H19ClN4O.2HCl
Storage
Desiccate at RT
Purity
≥99% (HPLC)
Chemical Name
8-Chloro-11-(4-methyl-4-oxido-1-piperazinyl)-5H-dibenzo[b,e][1,4]diazepine dihydrochloride
CAS Number
2250025-93-3
PubChem ID
137187247
InChI Key
MBRGKRXDVKTUPT-UHFFFAOYSA-N
SMILES
ClC(C=C1)=CC2=C1NC(C=CC=C3)=C3C(N4CC[N+](C)([O-])CC4)=N2.Cl.Cl
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 41.57 | 100 | |
| DMSO | 41.57 | 100 |
Preparing Stock Solutions
for Clozapine N-oxide dihydrochloride
The following data is based on the product molecular weight 415.74
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.41 mL | 12.03 mL | 24.05 mL |
| 5 mM | 0.48 mL | 2.41 mL | 4.81 mL |
| 10 mM | 0.24 mL | 1.20 mL | 2.41 mL |
| 50 mM | 0.05 mL | 0.24 mL | 0.48 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Nakajima Gs-coupled GPCR signalling in AgRP neurons triggers sustained increase in food intake. Nat.Commun. 2016 PMID: 26743492
- Allen A comparative study of the pharmacokinetics of cloz. N-oxide and cloz. N-oxide hydrochloride salt in rhesus macaques. J.Pharmacol.Exp.Ther. 2019 PMID: 30523062
- Roth DREADDs for Neuroscientists. Neuron 2016 PMID: 26889809
- Gomez Chemogenetics revealed: DREADD occupancy and activation via converted cloz. Science 2017 PMID: 28774929
- Zaman LMO4 is essential for paraventricular hypothalamic neuronal activity and calcium channel expression to prevent hyperphagia. J.Neurosci. 2014 PMID: 24381275
- Ambruster Evolving the lock to fit the key to create a family of G protein-coupled receptors potently activated by an inert ligand. Proc.Natl.Acad.Sci.U.S.A. 2007 PMID: 17360345
- Eiermann The involvement of CYP1A2 and CYP3A4 in the metabolism of cloz. Br.J.Clin.Pharmacol. 1997 PMID: 9384460
Product Specific Notices for Clozapine N-oxide dihydrochloride
For research use only
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