Are R&D Systems Caspase Inhibitors irreversible?

Yes, the majority of R&D Systems Caspase Inhibitors have a Fluoromethyl ketone (FMK) functional group on the C-terminus of the peptide, and act as effective irreversible inhibitors with no added cytotoxic effects. Inhibitors synthesized with a benzyloxycarbonyl group (also known as BOC or Z) at the N-terminus and O-methyl side chains exhibit enhanced cellular permeability. R&D Systems also offers a General Caspase Inhibitor, Q-VD-OPh, Catalog # OPH001, as well as a FITC-conjugated pan-caspase inhibitor (ApoStat), Catalog # FMK012, which are both cell-permeable, irreversible inhibitors of caspase activity.

Does R&D Systems offer a negative control for Caspase Inihibitors with benzyloxycarbonyl group (Z-) at the N-terminus and the FMK functional group at the C-terminus?

Yes, R&D Systems offers Caspase Inhibitor Control Z-FA-FMK, Catalog # FMKC01, which is an inhibitor of cathepsins B and L but not caspases, and has been used in several systems as a negative control for peptide inhibitors of caspases.

Caspase-3 Inhibitor Z-DEVD-FMK

Name: Caspase-3 Inhibitor Z-DEVD-FMK
Brand: R&D Systems
SKU: FMK004

R&D Systems, part of Bio-Techne | Catalog # FMK004

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Product Datasheet / COA / SDS

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Preparation & Storage
Background
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Specific Notices

Key Product Details

Cell permeable fluoromethyl ketone (FMK)-derivatized peptides act as effective irreversible Caspase inhibitors with no cytotoxic effects and, therefore, are useful tools for studying Caspase activity.

Conjugate

Unconjugated

View all Caspase-3 Small Molecules and Peptides »

Formulation, Preparation and Storage

FMK004

Background: Caspase-3

Caspase-3 is a widely expressed dimeric peptidase that is the major executioner caspase, the primary downstream mediator of apoptotic-associated proteolysis. Caspase-3 cleaves multiple substrates including PARP, proIL-16, PKC-gamma and -delta, proCaspases-6, -7, and -9, and beta-Catenin. Normally, it is an inactive, exclusively cytosolic homodimer. During apoptosis, however, proCaspase-3 is activated by cleavage into p20 and p12 subunits, and the p20 subunit is trimmed to yield a p17 subunit. Active Caspase-3 contains two p17 and two p12 subunits.

Alternate Names

Apopain, CASP3, Caspase3, CPP32, LICE-1, YAMA

Entrez Gene IDs

836 (Human); 12367 (Mouse)

Gene Symbol

CASP3

Additional Caspase-3 Products

Product Documents for Caspase-3 Inhibitor Z-DEVD-FMK

Product Datasheets

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COA

SDS

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Product Specific Notices for Caspase-3 Inhibitor Z-DEVD-FMK

For research use only

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