CAM 833

Name: CAM 833
Brand: Tocris Bioscience
SKU: 7457

Tocris Bioscience, part of Bio-Techne | Catalog # 7457

Citations (3)

COA / SDS

Selective orthosteric inhibitor of the BRCA2-RAD51 interaction

Key Product Details

Description:	Selective orthosteric inhibitor of the BRCA2-RAD51 interaction
Chemical Name:	N-(2-((2S,4R)-2-(((S)-1-(2-Chloro-4-methoxyphenyl)ethyl)carbamoyl)-4-hydroxypyrrolidin-1-yl)-2-oxoethyl)-6-fluoroquinoline-2-carboxamide
Purity:	≥98% (HPLC)
Molecular Weight:	528.97

View Full Technical Details for CAM 833

Technical Data
Citations

Biological Activity for CAM 833

CAM 833 a selective orthosteric inhibitor of the BRCA2-RAD51 interaction (K_dof 366 nM; IC₅₀ of 6 μM). CAM 833 has no significant off-target interactions when screened at 10 μM in the Cerep ExpresSPanel. It inhibits DNA recombinase RAD51-mediated homologous recombination through binding to RAD51 at the same site as the BRCA2 FxxA motif. In cells, CAM 833 suppresses the assembly of RAD51 into damage-induced filaments at the sites of DNA damage. CAM 833 potentiates radiation-induced cytotoxicity and poly-ADP ribose polymerase (PARP)1 inhibitor-induced growth suppression in BRCA2-wild-type cells. The product is metabolically stable, does not significantly inhibit CYP450 enzymes, and is suitable for in vivo investigation.

Technical Data for CAM 833

M. Wt	528.97
Formula	C₂₆H₂₆ClFN₄O₅
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	2758364-02-0
InChi Key	PMUWBFKMLGLUTF-KNUWZQJKSA-N
Smiles	FC1=CC2=CC=C(N=C2C=C1)C(NCC(N3[C@@H](C[C@H](C3)O)C(N[C@H](C4=C(C=C(C=C4)OC)Cl)C)=O)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for CAM 833

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	52.9	100
	ethanol	2.64	5

Preparing Stock Solutions for CAM 833

The following data is based on the product molecular weight 528.97.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.89 mL	9.45 mL	18.90 mL
5 mM	0.38 mL	1.89 mL	3.78 mL
10 mM	0.19 mL	0.95 mL	1.89 mL
50 mM	0.04 mL	0.19 mL	0.38 mL

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References for CAM 833

References are publications that support the biological activity of the product.

Scott A small-molecule inhibitor of the BRCA2-RAD51 interaction modulates RAD51 assembly and potentiates DNA damage-induced cell death. Cell Chem.Biol. 2021 PMID: 33662256