BromoCatch™ Control Ligand

Name: BromoCatch™ Control Ligand
Brand: Tocris Bioscience
SKU: 7300

Tocris Bioscience, part of Bio-Techne | Catalog # 7300

COA / SDS

Control ligand for use with BromoCatch™, a Self-Labeling Proteins (SLPs) tag platform

Key Product Details

Description:	Control ligand for use with BromoCatch™, a Self-Labeling Proteins (SLPs) tag platform
Alternative Names:	Et-JQ1-OMe
Chemical Name:	Methyl (R)-2-((S)-4-(4-chlorophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)butanoate
Purity:	≥97% (HPLC)
Molecular Weight:	442.96

View Full Technical Details for BromoCatch™ Control Ligand

Technical Data
Citations

Biological Activity for BromoCatch™ Control Ligand

BromoCatch™ Control Ligand is a competitor ligand for use with BromoCatch™, a Self-Labeling Proteins (SLPs) tag platform. It is a non-covalent ligand that competes for BromoCatch™ probe binding sites to validate the specificity of the BromoCatch™ system in your experiments, ensuring that the interactions observed are specific to the target probe. It is particularly useful in experimental settings like fluorescence microscopy and biochemical assays where confirmation of probe specificity is crucial.

Please refer to this protocol for further information on how to use this product.

BromoCatch is a trademark of Bio-Techne Corp.

Technical Data for BromoCatch™ Control Ligand

M. Wt	442.96
Formula	C₂₂H₂₃ClN₄O₂S
Storage	Store at -20°C
Purity	≥97% (HPLC)
CAS Number	2421153-75-3
PubChem ID	146037626
InChi Key	WLKVOOMXHSBIOW-APWZRJJASA-N
Smiles	CC1=C(C)C(C(C2=CC=C(C=C2)Cl)=N[C@@H]([C@H](C(OC)=O)CC)C3=NN=C(C)N43)=C4S1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for BromoCatch™ Control Ligand

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	44.3	100
	ethanol	44.3	100

Preparing Stock Solutions for BromoCatch™ Control Ligand

The following data is based on the product molecular weight 442.96.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.26 mL	11.29 mL	22.58 mL
5 mM	0.45 mL	2.26 mL	4.52 mL
10 mM	0.23 mL	1.13 mL	2.26 mL
50 mM	0.05 mL	0.23 mL	0.45 mL

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References for BromoCatch™ Control Ligand

References are publications that support the biological activity of the product.

Baud A bump-and-hole approach to engineer controlled selectivity of BET bromodomain chemical probes. Science 2014 PMID: 25323695
Rodriguez-Rios BromoCatch: a self-labelling tag platform for protein analysis and live cell imaging. biorxiv
Runcie Optimization of a "bump-and-hole" approach to allele-selective BET bromodomain inhibition. Chem.Sci. 2018 PMID: 29732121