Biotin-PEG4-Diazirine
Tocris Bioscience, part of Bio-Techne | Catalog # 8994
Photo-crosslinking capture probe for deazaflavin-diazirine energy-transfer labelling (DarT labelling) with a biotin handle
Key Product Details
Description
Photo-crosslinking capture probe for deazaflavin-diazirine energy-transfer labelling (DarT labelling) with a biotin handle
Product Description
Key information:Biotin-PEG4-Diazirine is a cell-permeable photo-crosslinking probe for elucidating protein interactome of polyarginine cell-penetrating peptides and mapping cell surfaceome through deazaflavin-diazirine energy-transfer labelling (DarT labelling). Upon UV irradiation and in the presence of deazaflavin as an organic photocatalyst, the diazirine group is activated to a reactive carbene that covalently crosslinks to nearby proteins. The appended biotin handle allows for efficient affinity-based detection, pull-down, and isolation of tagged proteins from complex mixtures such as cell lysates. Suitable for live-cell applications.Used for: photocatalytic proximity labelling.
Application: deazaflavin-diazirine energy-transfer labelling (DarT labelling) followed by affinity-based detection, pull-down, and isolation of tagged proteins from complex mixtures.
Product Specifications
Molecular Weight
688.75
Formula
C30H43F3N6O7S
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
1-(5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)-N-(4-(3-(trifluoromethyl)-3H-diazirin-3-yl)benzyl)-3,6,9,12-tetraoxapentadecan-15-amide
InChI Key
MGUZAQIDWYWQHB-DPZBCOQUSA-N
SMILES
O=C1N[C@@]([H])([C@]([H])(CS2)N1)[C@@H]2CCCCC(NCCOCCOCCOCCOCCC(NCC3=CC=C(C4(N=N4)C(F)(F)F)C=C3)=O)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 34.44 | 50 with gentle warming |
Preparing Stock Solutions
for Biotin-PEG4-Diazirine
The following data is based on the product molecular weight 688.75
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 2.90 mL | 14.52 mL | 29.04 mL |
| 2.5 mM | 0.58 mL | 2.90 mL | 5.81 mL |
| 5 mM | 0.29 mL | 1.45 mL | 2.90 mL |
| 25 mM | 0.06 mL | 0.29 mL | 0.58 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Crocker Energy-transfer photoproximity labelling in live cells using an organic cofactor. Nature Chemistry 2025 PMID: 40962911
Product Specific Notices for Biotin-PEG4-Diazirine
For research use only
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